Mouse Macrophage Inflammatory Protein 1β,MIP-1β ELISA kit

Instructions
Code CSB-E04665m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-C motif) ligand 4
Alternative Names Ccl4 ELISA Kit; Mip1b ELISA Kit; Scya4 ELISA Kit; C-C motif chemokine 4 ELISA Kit; Immune activation protein 2 ELISA Kit; ACT-2 ELISA Kit; ACT2 ELISA Kit; Macrophage inflammatory protein 1-beta ELISA Kit; MIP-1-beta ELISA Kit; Protein H400 ELISA Kit; SIS-gamma ELISA Kit; Small-inducible cytokine A4 ELISA Kit
Abbreviation CCL4
Uniprot No. P14097
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human EPHB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.    
    Sample   Serum(n=4)      
1:1   Average %   102      
    Range %   98-106      
1:2   Average %   85      
    Range %   80-90      
1:4   Average %   100      
    Range %   97-103      
1:8   Average %   86      
    Range %   83-89      
Recovery
The recovery of human EPHB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
 
Sample Type   Average % Recovery   Range      
Serum (n=5)   99   95-103      
EDTA plasma (n=4)   87   83-90      
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected      
4000 2.409 2.433 2.421 2.262      
2000 1.778 1.659 1.719 1.560      
1000 1.216 1.268 1.242 1.083      
500 0.861 0.835 0.848 0.689      
250 0.616 0.535 0.576 0.417      
125 0.432 0.435 0.434 0.275      
62.5 0.296 0.289 0.293 0.134      
0 0.159 0.158 0.159        
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Monokine with inflammatory and chemokinetic properties.
Gene References into Functions
  1. CCL4-CCR5 axis can contribute to breast cancer metastasis to bone by mediating the interaction between cancer cells and fibroblasts in bone cavity. PMID: 27177471
  2. YY1 regulates lung CCL4 transcription in pulmonary tuberculosis. PMID: 26786659
  3. Ccl4 mRNAs increased within 5 h after injury in mouse cortical slices. PMID: 25895671
  4. CCL4 and CCL5 expression was higher in livers of infected WSX-1(-/-) mice than infected WT mice, and hepatic CD4 T cells from WSX-1(-/-) mice expressed higher levels of CCR5 than cells from WT mice PMID: 24244314
  5. CCL4 upregulation was associated with increased Snail expression and downregulation of p53/PTEN in high-grade PIN and prostate cancer PMID: 23878190
  6. These results suggest that MIP-1beta is a novel key mediator, and the peripheral MIP-1beta-CCR5 axis contributes to neuropathic pain. PMID: 22528550
  7. results indicate that MIP-1beta is involved in the recruitment of bone marrow-derived monocyte lineage cells PMID: 21912378
  8. increased responsiveness of murine eosinophils to MIP-1beta is mediated by CCR5 receptor in mice PMID: 12050188
  9. macrophage inflammatory protein-1beta is a hypoxia-induced neutrophil survival factor. PMID: 15630139
  10. MIP-1beta is overexpressed, and VE-cadherin is underexpressed in heart transplant allografts compared with isografts PMID: 15897346
  11. Overproduction of MIP-1beta in chemokine (C-C motif) receptor 5-deficient mice after collagen II-immunization may contribute partially to the occurrence of arthritis PMID: 15967376
  12. The expression and role CXCL12 and CCL4 and their receptors (CXCR4 and CCR5) in regulating thymocyte migration in conjunction with extracellular matrix during acute T. cruzi infection are investigated. PMID: 16637021
  13. ATF3 appears to be part of a control mechanism that limits the amount of CCL4 released by macrophages, preventing excessive inflammation. PMID: 16982098
  14. Antibody neutralization of CCL4 abrogates the ability of T-cells from IL-4-treated NOD mice to transfer protection against type 1 diabetes. PMID: 17327452

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Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: mmu:20303

STRING: 10090.ENSMUSP00000019074

UniGene: Mm.244263

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