Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 20-100 ng/ml.

Target Names

Ccl4

Uniprot No.

P14097

Research Area

Immunology

Alternative Names

Ccl4; Mip1b; Scya4; C-C motif chemokine 4; Immune activation protein 2; ACT-2; ACT2; Macrophage inflammatory protein 1-beta; MIP-1-beta; Protein H400; SIS-gamma; Small-inducible cytokine A4

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

24-92aa

Complete Sequence

APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN

Mol. Weight

7.8 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered 2X PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CCL4 (MIP-1-beta) is a key CC chemokine that drives monocyte and lymphocyte recruitment to inflammatory sites, and this tag-free recombinant mouse protein enables direct investigation of that biology with high confidence. Validated in a human monocyte chemotaxis bioassay at an active concentration range of 20–100 ng/ml, it provides a suitable basis for Transwell migration assays, CCR5 signaling pathway studies involving MAPK and NF-κB activation, and in vivo mouse models of inflammation or infection. The construct encompasses the full-length mature sequence (residues 24–92) expressed in *E. coli*, preserving native receptor-binding fidelity without tag-related steric interference — an important consideration when using the protein as an immunogen for antibody development or as a reference standard in cytokine detection ELISAs. Purity exceeding 97% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the stringent criteria needed to prevent LPS-driven artifacts in sensitive immune cell functional assays.

Target Background

Function

Monokine with inflammatory and chemokinetic properties.

Gene References into Functions

CCL4-CCR5 axis can contribute to breast cancer metastasis to bone by mediating the interaction between cancer cells and fibroblasts in bone cavity. PMID: 27177471
YY1 regulates lung CCL4 transcription in pulmonary tuberculosis. PMID: 26786659
Ccl4 mRNAs increased within 5 h after injury in mouse cortical slices. PMID: 25895671
CCL4 and CCL5 expression was higher in livers of infected WSX-1(-/-) mice than infected WT mice, and hepatic CD4 T cells from WSX-1(-/-) mice expressed higher levels of CCR5 than cells from WT mice PMID: 24244314
CCL4 upregulation was associated with increased Snail expression and downregulation of p53/PTEN in high-grade PIN and prostate cancer PMID: 23878190
These results suggest that MIP-1beta is a novel key mediator, and the peripheral MIP-1beta-CCR5 axis contributes to neuropathic pain. PMID: 22528550
results indicate that MIP-1beta is involved in the recruitment of bone marrow-derived monocyte lineage cells PMID: 21912378
increased responsiveness of murine eosinophils to MIP-1beta is mediated by CCR5 receptor in mice PMID: 12050188
macrophage inflammatory protein-1beta is a hypoxia-induced neutrophil survival factor. PMID: 15630139
MIP-1beta is overexpressed, and VE-cadherin is underexpressed in heart transplant allografts compared with isografts PMID: 15897346
Overproduction of MIP-1beta in chemokine (C-C motif) receptor 5-deficient mice after collagen II-immunization may contribute partially to the occurrence of arthritis PMID: 15967376
The expression and role CXCL12 and CCL4 and their receptors (CXCR4 and CCR5) in regulating thymocyte migration in conjunction with extracellular matrix during acute T. cruzi infection are investigated. PMID: 16637021
ATF3 appears to be part of a control mechanism that limits the amount of CCL4 released by macrophages, preventing excessive inflammation. PMID: 16982098
Antibody neutralization of CCL4 abrogates the ability of T-cells from IL-4-treated NOD mice to transfer protection against type 1 diabetes. PMID: 17327452

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Database Links

KEGG: mmu:20303

STRING: 10090.ENSMUSP00000019074

UniGene: Mm.244263

Code	CSB-AP001231MO
Abbreviation	Recombinant Mouse Ccl4 protein (Active)
MSDS	MSDS Datasheet
Size	$354
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Recombinant Mouse C-C motif chemokine 4 protein (Ccl4) (Active)

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Target Background

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