Mouse Macrophage Inflammatory Protein 1β,MIP-1β ELISA kit

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Mouse Macrophage Inflammatory Protein 1β,MIP-1β ELISA kit

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Protocol

Code	CSB-E04665m-IS
Size	96T,5×96T,10×96T
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Alternative Names

Ccl4 ELISA Kit; Mip1b ELISA Kit; Scya4 ELISA Kit; C-C motif chemokine 4 ELISA Kit; Immune activation protein 2 ELISA Kit; ACT-2 ELISA Kit; ACT2 ELISA Kit; Macrophage inflammatory protein 1-beta ELISA Kit; MIP-1-beta ELISA Kit; Protein H400 ELISA Kit; SIS-gamma ELISA Kit; Small-inducible cytokine A4 ELISA Kit

Abbreviation

CCL4

Uniprot No.

P14097

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIP-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	102
	Range %	95-108
1:2	Average %	96
	Range %	91-102
1:4	Average %	91
	Range %	87-96
1:8	Average %	90
	Range %	86-96

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

200

2.483

2.634

2.558

2.386

100

1.530

1.617

1.574

1.401

1.009

0.957

0.983

0.811

0.557

0.529

0.543

0.371

12.5

0.350

0.331

0.340

0.168

6.25

0.244

0.253

0.249

0.076

3.12

0.196

0.184

0.190

0.018

0.177

0.168

0.172

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Description

This kit provides quantitative measurement of CCL4 (MIP-1β) in mouse samples, supporting immunology research focused on chemokine-driven leukocyte recruitment and inflammatory signaling. Because CCL4 is rapidly secreted at low concentrations during early immune activation and varies dramatically between resting and stimulated conditions, precise quantification across diverse sample contexts is essential.

Detection Range: The 3.12–200 pg/mL range spans CCL4 levels from baseline circulating concentrations in naive mouse serum to elevated levels observed during acute inflammation or LPS challenge. Most stimulated samples will fall within range without dilution, though heavily inflamed tissue homogenates may require pre-dilution.

Sensitivity: At 1.56 pg/mL, the kit detects CCL4 in unstimulated macrophage supernatants and resting-state serum, where concentrations often fall in the single-digit pg/mL range — critical for establishing true baseline values before immune challenge.

Sample Compatibility: Validation across serum, plasma, cell culture supernatants, and tissue homogenates enables researchers to correlate systemic CCL4 levels with local tissue production and in vitro macrophage or T cell secretion within a single experimental framework.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate limited-volume specimens from mouse bleeds and small-scale primary cell cultures typical of adoptive transfer or bone marrow-derived macrophage experiments.

This kit is particularly suited for studies of viral infection-driven inflammation, tumor microenvironment immune profiling, and CCR5-dependent T cell trafficking.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Monokine with inflammatory and chemokinetic properties.

Gene References into Functions

CCL4-CCR5 axis can contribute to breast cancer metastasis to bone by mediating the interaction between cancer cells and fibroblasts in bone cavity. PMID: 27177471
YY1 regulates lung CCL4 transcription in pulmonary tuberculosis. PMID: 26786659
Ccl4 mRNAs increased within 5 h after injury in mouse cortical slices. PMID: 25895671
CCL4 and CCL5 expression was higher in livers of infected WSX-1(-/-) mice than infected WT mice, and hepatic CD4 T cells from WSX-1(-/-) mice expressed higher levels of CCR5 than cells from WT mice PMID: 24244314
CCL4 upregulation was associated with increased Snail expression and downregulation of p53/PTEN in high-grade PIN and prostate cancer PMID: 23878190
These results suggest that MIP-1beta is a novel key mediator, and the peripheral MIP-1beta-CCR5 axis contributes to neuropathic pain. PMID: 22528550
results indicate that MIP-1beta is involved in the recruitment of bone marrow-derived monocyte lineage cells PMID: 21912378
increased responsiveness of murine eosinophils to MIP-1beta is mediated by CCR5 receptor in mice PMID: 12050188
macrophage inflammatory protein-1beta is a hypoxia-induced neutrophil survival factor. PMID: 15630139
MIP-1beta is overexpressed, and VE-cadherin is underexpressed in heart transplant allografts compared with isografts PMID: 15897346
Overproduction of MIP-1beta in chemokine (C-C motif) receptor 5-deficient mice after collagen II-immunization may contribute partially to the occurrence of arthritis PMID: 15967376
The expression and role CXCL12 and CCL4 and their receptors (CXCR4 and CCR5) in regulating thymocyte migration in conjunction with extracellular matrix during acute T. cruzi infection are investigated. PMID: 16637021
ATF3 appears to be part of a control mechanism that limits the amount of CCL4 released by macrophages, preventing excessive inflammation. PMID: 16982098
Antibody neutralization of CCL4 abrogates the ability of T-cells from IL-4-treated NOD mice to transfer protection against type 1 diabetes. PMID: 17327452

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Database Links

KEGG: mmu:20303

STRING: 10090.ENSMUSP00000019074

UniGene: Mm.244263

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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