Mouse Mucin-5 subtype AC,MUC5AC ELISA Kit

Code CSB-E11040m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse MUC5AC ELISA Kit was designed for the quantitative measurement of Mouse MUC5AC protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.688 ng/mL-300 ng/mL and the sensitivity is 1.172 ng/mL.

Target Name Mucin-5 subtype AC,MUC5AC
Abbreviation MUC5AC
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 4.688 ng/mL-300 ng/mL
Sensitivity 1.172 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %82-92
1:2Average %90
Range %85-95
1:4Average %98
Range %91-102
1:8Average %96
Range %93-103
Recovery
The recovery of mouse MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9891-104
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
3002.275 2.182 2.229 2.057
1501.463 1.434 1.449 1.277
750.855 0.818 0.837 0.665
37.50.524 0.507 0.516 0.344
18.750.351 0.329 0.340 0.168
9.3750.281 0.262 0.272 0.100
4.6880.235 0.217 0.226 0.054
00.174 0.169 0.172  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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