Mouse Mucin-5 subtype AC,MUC5AC ELISA Kit

Mouse Mucin-5 subtype AC,MUC5AC ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Mouse Mucin-5 subtype AC,MUC5AC ELISA Kit

Name: Mouse Mucin-5 subtype AC,MUC5AC ELISA Kit
Brand: CUSABIO
SKU: CSB-E11040m

Citations (8) Protocol MSDS

Code	CSB-E11040m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details
Citations (8)
Related Products
Customer Reviews and Q&A

Target Name

Mucin-5 subtype AC,MUC5AC

Abbreviation

MUC5AC

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

4.688 ng/mL-300 ng/mL

Sensitivity

1.172 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	90
	Range %	85-95
1:4	Average %	98
	Range %	91-102
1:8	Average %	96
	Range %	93-103

Recovery

The recovery of mouse MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	91-104
EDTA plasma (n=4)	95	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.275	2.182	2.229	2.057
150	1.463	1.434	1.449	1.277
75	0.855	0.818	0.837	0.665
37.5	0.524	0.507	0.516	0.344
18.75	0.351	0.329	0.340	0.168
9.375	0.281	0.262	0.272	0.100
4.688	0.235	0.217	0.226	0.054
0	0.174	0.169	0.172

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse MUC5AC ELISA Kit was designed for the quantitative measurement of Mouse MUC5AC protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.688 ng/mL-300 ng/mL and the sensitivity is 1.172 ng/mL.

Discovery of Fungus-Derived Nornidulin as a Novel TMEM16A Inhibitor: A Potential Therapy to Inhibit Mucus Secretion in Asthma P Pongkorpsakol,Journal of experimental pharmacology,2023
Gleditsia sinensis Lam. aqueous extract attenuates nasal inflammation in allergic rhinitis by inhibiting MUC5AC production through suppression of the STAT3/STAT6 pathway MA Jung,Biomedicine & pharmacotherapy,2023
Alnus hirsuta (Spach) Rupr. Attenuates Airway Inflammation and Mucus Overproduction in a Murine Model of Ovalbumin-Challenged Asthma Ji-Hye Ha,Frontiers in Pharmacology,2021
Combined treatment with SB203580 and dexamethasone suppresses non-typeable Haemophilus influenzae-induced Th17 inflammation response in murine allergic asthma Wang G, et al,European Journal of Pharmacology,2019
Chronic intermittent hypoxiaDecreases pulmonary clearance of 99mTc-labelled particulate matter in mice Fu C.et al,Am J Transl Res,2017
The immune profile associated with acute allergic asthma accelerates clearance of influenza virus Samarasinghe AE et al,Immunol Cell Biol,2014
A Dissociated Glucocorticoid Receptor Modulator Reduces Airway Hyperresponsiveness and Inflammation in a Mouse Model of Asthma /,The Journal of Immunology,2012
Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice /,PLOS ONE,2012

■ Customer Reviews

Average Rating:

5.0 - 3 reviews

Submit a Review here

Sample type: Tissue homogenate

Sample species: Mouse

Sample dilution: No dilution

Review: I have tested the CSB-E11040m for PR8 virus-infected mouse lung tissue homogenates, the operation is simple and easy to use, the sensitivity is very high, and the test results are very accurate.

By Anonymous

Sample type: Tissue homogenate(lung tissue homogenate supernatant)

Sample species: Mouse

Sample dilution: 1:9

Review: I have detected influenza virus infection in the lung tissue homogenate supernatant of mice using CSB-E11040m. The operation is simple, convenient, and easy to get started, with very high sensitivity and very accurate detection results.

By Anonymous

Sample type: Biological fluids(bronchoalveolar lavage fluid)

Sample species: Mouse

Sample dilution: No dilution

Review: MUC5AC levels in bronchoalveolar lavage fluid (BALF) of normal and smoking C57B/L mice were detected by CSB-E11040m. The OD values of normal mice ranged from 0.061 to 0.415, and those of smoking mice ranged from 0.652 to 0.776. Simple and convenient operation, recommended!

By Anonymous

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.