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| Code | CSB-EL017255MO |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSCAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 89 | ||||
| Range % | 82-93 | |||||
| 1:10 | Average % | 105 | ||||
| Range % | 97-108 | |||||
| 1:20 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| The recovery of mouse OSCAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 91 | 85-94 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Mouse Osteoclast-associated immunoglobulin-like receptor (OSCAR) ELISA kit is a reliable and accurate tool for the detection of OSCAR protein levels in mouse serum, plasma, and tissue homogenates. OSCAR is a type I transmembrane protein that is predominantly expressed on osteoclasts and plays a crucial role in bone resorption.
The Mouse OSCAR ELISA kit has a wide detection range of 37.5 pg/mL-2400 pg/mL and a high sensitivity of 9.375 pg/mL, ensuring precise and accurate measurements even at low concentrations. The kit uses a sandwich assay principle, which involves the use of two monoclonal antibodies specific to the mOSCAR protein.
The assay time for the kit is 1-5 hours, and the sample volume required is 50-100ul. Detection is done at a wavelength of 450 nm, making it easy to read and interpret the results. The kit is suitable for use in immunology research, where OSCAR protein levels are of interest, and has been cited in over 2 research articles.
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