Mouse P-Selectin ELISA kit

Instructions
Code CSB-E04709m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name selectin P (granule membrane protein 140kDa, antigen CD62)
Alternative Names Selp ELISA Kit; GrmpP-selectin ELISA Kit; CD62 antigen-like family member P ELISA Kit; Granule membrane protein 140 ELISA Kit; GMP-140 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 3 ELISA Kit; LECAM3 ELISA Kit; Platelet activation dependent granule-external membrane protein ELISA Kit; PADGEM ELISA Kit; CD antigen CD62P ELISA Kit
Abbreviation SELP
Uniprot No. Q01102
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse P-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 92  
Range % 84-98  
1:200 Average % 95  
Range % 81-101  
1:400 Average % 97  
Range % 93-103  
1:800 Average % 95  
Range % 89-98  
Recovery
The recovery of mouse P-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-99  
EDTA plasma (n=4) 97 95-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.768 2.710 2.739 2.568  
1000 2.263 2.224 2.244 2.073  
500 1.576 1.557 1.567 1.396  
250 1.026 1.004 1.015 0.844  
125 0.595 0.611 0.603 0.432  
62.5 0.441 0.438 0.440 0.269  
31.25 0.289 0.271 0.280 0.109  
0 0.169 0.172 0.171    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.
Gene References into Functions
  1. In a laser injury-induced thrombosis model, P-selectin modulates thrombus propagation independently of VWF and TSP1 PMID: 27943541
  2. deletion of P-sel disrupted megakaryocyte/neutrophil interactions in spleen, reduced TGF-beta content, and corrected the hematopoietic stem cells distribution that in Gata1(low) mice, as in primary myelofibrosis patients, is abnormally expanded in spleen. PMID: 26439305
  3. The present study supported the notion that CD62P accumulation during platelet storage is involved in mediating transfusion-related acute lung injury. PMID: 26397744
  4. Holothurian glycosaminoglycan has emerged as a novel anti-cancer agent via blocking P-selectin-mediated malignant events of tumor metastasis. PMID: 26318439
  5. Data (including data from studies in transgenic mice) suggest that sequence variations limited to 1.4-kb proximal promoter region account for most differences in expression of murine Selp and human SELP genes. PMID: 26631722
  6. P-selectin has a role in endothelial progenitor cells' inhibition of platelet function PMID: 25948279
  7. Both platelet and endothelial cell P-selectin contribute to host defense during Klebsiella pneumosepsis. PMID: 25773400
  8. Platelet infiltration into solid tumors occurs through a P-selectin-dependent mechanism and promotes tumor growth. PMID: 25762641
  9. P-selectin promotes neutrophil extracellular trap (NET) formation and is a potential target for NET-related diseases. PMID: 25979951
  10. P-selectin is an important adhesion molecule vital for infiltration of regulatory T cells into mouse mammary tumors PMID: 25865803
  11. Administering human recombinant PTX3 to wild-type mice induced endothelial dysfunction and increased blood pressure, an effect absent in P-selectin-deficient mice. PMID: 25747934
  12. Targeting P-selectin-mediated platelet adhesion onto endometriotic lesions holds promise as a novel therapeutics for treating endometriosis. PMID: 25681855
  13. Endothelial peroxisome proliferator-activated receptor-gamma protects against thrombosis through a mechanism that involves downregulation of P-selectin expression and diminished P-selectin-mediated leukocyte-endothelial interactions. PMID: 25675995
  14. These results demonstrate that chronically raised sP-selectin favours progression of an unstable atherosclerotic plaque phenotype. PMID: 24846287
  15. P-selectin-mediated platelet adhesion promotes the metastasis of murine melanoma cells. PMID: 24632801
  16. (68)Ga-Fucoidan represents a versatile imaging biomarker for P-selectin with the potential to specifically detect P-selectin expression using positron emission tomography and to discriminate vulnerable plaques in vivo. PMID: 24903095
  17. The ability of ascorbate to reduce platelet aggregation and P-selectin expression could be an important mechanism by which ascorbate inhibits capillary plugging in sepsis. PMID: 23402318
  18. These experiments define a novel VEGF-miR-1-Mpl-P-selectin effector pathway in lung Th2 inflammation and herald the utility of miR-1 and Mpl as potential therapeutic targets for asthma. PMID: 24043765
  19. These results demonstrated the ability for Abeta to enhance p-selectin expression at the cerebral endothelial cell surface and induce cytoskeleton reorganization. PMID: 23593361
  20. Expression of P-selectin is sufficient to support rescue & hematopoietic reconstitution of lethally irradiated recipients forllowing bone marrow transplant. PMID: 23499761
  21. These results suggest that AT1A signaling plays a critical role in tumor metastasis through P-selectin-mediated interactions of platelets with tumor and endothelial cells. PMID: 23219751
  22. Older animals had significantly larger venous thrombi versus younger animals post-thombosis, as a result of high levels of P-selectin both in the circulation and locally at the level of the vein wall. PMID: 23174624
  23. P-selectin is a candidate target molecule for early-phase detection by PET and CT fusion imaging of atherosclerotic plaques. PMID: 23485468
  24. our findings help clarify the roles of platelets, NK cells and P-selectin in metastasis PMID: 22836751
  25. the first evidence for P-selectin upregulation by persistent mCMV infection and implicate this adhesion molecule in the associated arteriolar dysfunction. P-selectin PMID: 21457388
  26. Loss of CD63 has a similar phenotype to loss of P-selectin itself, thus CD63 is an essential cofactor to P-selectin. PMID: 21803846
  27. Data show that Tpst1/Tpst2 DKO leukocytes bound less P-selectin than wild type leukocytes despite equivalent surface expression of Psgl-1. PMID: 21633705
  28. Demonstrate a profound estrogen-dependent suppression of leukocyte recruitment in females via reduced endothelial P-selectin expression. PMID: 21350195
  29. P-selectin and E-selectin mediate recruitment of reperfusion-induced bone marrow stromal cells to the infarct site, thereby enabling their adhesion to the postischemic cerebral microvasculature. PMID: 21257828
  30. Findings reveal functionally significant differences in basal and inducible expression of human and murine P-selectin in vivo. PMID: 21149548
  31. P-selectin is important in host innate immune response against Citrobacter infection. PMID: 20345490
  32. Suggest that platelet binding activates neutrophils and contributes to a chronic inflammatory state and pulmonary dysfunction in sickle cell disease via P-selectin. PMID: 21071696
  33. P-selectin-deficient allo-bone marrow transplant recipients are resistant to the development of graft-versus-host disease (GVHD), which suggests that P-selectin of recipient origin is an important molecule for GVHD pathophysiology. PMID: 20622117
  34. Results reveal an intracellular WPb-independent P-selectin pool in pulmonary capillary endothelium, where the regulated P-selectin surface expression is triggered by Ca(2+) transients evoked through activation of the alpha(1G) T-type channel. PMID: 20435690
  35. Platelet, not endothelial, P-selectin contributes to generation of immunity in 2,4,-dinitrofluorobenzene-induced contact hypersensitivity. PMID: 20056837
  36. P-selectin and P-selectin glycoprotein ligand 1 are dominating molecules in supporting adhesive interactions of CD8 T cells in inflamed colonic venules PMID: 19730132
  37. Dual targeting improves microbubble contrast agent adhesion to VCAM-1 and P-selectin under flow. PMID: 19666063
  38. PL-selectin double deficient mice show that the two selectins work synergistically. PMID: 11854515
  39. P-selectin may be a key target to reduce pathological recruitment of inflammatory cells in the colon. PMID: 11934816
  40. The N-terminal peptide of PSGL-1 can mediate adhesion to trauma-activated endothelium via P-selectin in vivo. PMID: 12091345
  41. The role of P-selectin overlaps that of alpha 4 integrin in mediating leukocyte-endothelial cell interactions during experimental autoimmune encephalomyelitis. PMID: 12097407
  42. P-selectin is incolved in the development of Con A-induced liver injury and reveal the protective effect of blocking P-selectin in this hepatitis. PMID: 12149416
  43. Early CNS migration of a CD4 Th1 cell line was facilitated by P selectin in (PL/JxSJL/J)F1 mice. Genetically determined kinetics of immune surveillance may regulate the phenotype of subsequent CNS inflammation. PMID: 12161020
  44. platelet P-selectin is required for monocyte infiltration during neointima formation in injured carotid arteries of apoE-/- mice PMID: 12234959
  45. The migration of in-vitro generated cytotoxic T lymphocytes into inflamed skin is partially mediated by P-selectin. PMID: 12370362
  46. A dysfunctional immune response in P-selectin knockout mice lacking also E-selectin results from impaired leukocyte trafficking and leads to chronic ulcerative dermatitis. PMID: 12391189
  47. data suggest that sulfation of Tyr13 and O-glycosylation of Thr17 are necessary for murine PSGL-1 to bind optimally to P-selectin PMID: 12393631
  48. endothelial P-selectin is critical for postischemic platelet-endothelial cell interactions within hepatic presinusoidal arterioles and postsinusoidal venules. P-selectin deficiency prevents microvascular injury and apoptosis after warm hepatic I/R PMID: 12462561
  49. the role of platelet versus endothelial P-selectin in generating sP-selectin and in the formation of atherosclerotic lesions PMID: 12480714
  50. P-selectin-mediated leukocyte-rolling velocity is stabilized by leukocyte deformation during rolling that offsets increased bond breakage at higher shear stress PMID: 12483549

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Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Selectin/LECAM family
Tissue Specificity Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Database Links

KEGG: mmu:20344

STRING: 10090.ENSMUSP00000123924

UniGene: Mm.3337

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