Mouse Pentosidine ELISA Kit

Code CSB-E09417m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details


This Mouse Pentosidine ELISA Kit was designed for the quantitative measurement of Mouse Pentosidine protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

Target Name Pentosidine
Abbreviation Pentosidine
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:100Average %96
Range %91-103
1:200Average %85
Range %80-90
1:400Average %93
Range %89-98
1:800Average %99
Range %94-105
The recovery of mouse pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-96
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
20002.401 2.462 2.432 2.251
10001.850 2.004 1.927 1.746
5001.352 1.432 1.392 1.211
2500.849 0.899 0.874 0.693
1250.601 0.650 0.626 0.445
62.50.447 0.417 0.432 0.251
31.250.285 0.274 0.280 0.099
00.183 0.178 0.181
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Regarding your ELISA kits CSB-E09417m, could you please advise on the following:
1) Are the standards natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Do the standards include additive agents such as antiseptic or preservative? If they include, What is composition of additive agent?
3) Material name and concentration of Stop Solution.
4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

Thanks for your inquiry.
CSB-E09417m Standard is purified Pentosidine polymer.
Preservative is porclin 300(1:3000).
Stop solution is 4N sulfuric acid;
These kits do not include hazardous substance such as azide, thimerosal, 2-ME, etc.
Pls let me know if you have any further questions. Thank you.
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7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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