Rat Pentosidine ELISA Kit

Instructions
Code CSB-E09416r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name Pentosidine
Abbreviation Pentosidine
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pmol/mL-1000 pmol/mL
Sensitivity 3.9 pmol/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %84
Range %80-89
1:2Average %89
Range %85-93
1:4Average %96
Range %92-100
1:8Average %92
Range %86-97
Recovery
The recovery of rat Pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10196-105
EDTA plasma (n=4)9085-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pmol/mlOD1OD2AverageCorrected
10002.065 2.051 2.058 1.966
5001.361 1.292 1.327 1.235
2500.745 0.697 0.721 0.629
1250.389 0.412 0.401 0.309
62.50.219 0.233 0.226 0.134
31.20.189 0.201 0.195 0.103
15.60.127 0.138 0.133 0.041
00.094 0.089 0.092  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

Regarding your ELISA kits CSB-E09416r, could you please advise on the following:
1) Are the standards natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Do the standards include additive agents such as antiseptic or preservative? If they include, What is composition of additive agent?
3) Material name and concentration of Stop Solution.
4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

A:
Thanks for your inquiry.
CSB-E09416r Standard is purified Pentosidine polymer.
Preservative is porclin 300(1:3000).
Stop solution is 4N sulfuric acid;
These kits do not include hazardous substance such as azide, thimerosal, 2-ME, etc.
Pls let me know if you have any further questions. Thank you.
 Customer Reviews

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