Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) ELISA kit

Code CSB-EL018425MO
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O70343
Lead Time 3-5 working days
Abbreviation PPARGC1A
Protein Biological Process 1 Transcription/Transcription regulation
Target Name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Alias LEM6, PGC-1(alpha), PGC-1v, PGC1, PGC1A, PPARGC1, PPAR gamma coactivator variant form|PPAR gamma coactivator-1|ligand effect modulator-6|peroxisome proliferative activated receptor, gamma, coactivat
Species Mus musculus (Mouse)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
HGNC 9237
RGD 620925
MGI 1342774
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PPARGC1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %86-96
1:2Average %104
Range %97-108
1:4Average %94
Range %87-98
1:8Average %99
Range %92-102
Recovery
The recovery of mouse PPARGC1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-97
EDTA plasma (n=4)9990-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.730 2.582 2.656 2.466
7502.181 2.076 2.129 1.939
3751.365 1.345 1.355 1.165
187.50.871 0.832 0.852 0.662
940.550 0.541 0.546 0.356
470.385 0.378 0.382 0.192
23.50.288 0.279 0.284 0.094
00.192 0.188 0.190
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Function Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK. Isoform 4 specifically activates the expression of IGF1 and suppresses myostatin expression in skeletal muscle leading to muscle fiber hypertrophy.
Subcellular Location Nucleus, Nucleus, PML body
Tissue Specificity White quadriceps and red tibialis anterior (TA) muscles, liver, kidney and brown adipose tissue (at protein level). Skeletal muscle, brown adipose tissue, heart, kidney and brain.
Database Links

KEGG: mmu:19017

STRING: 10090.ENSMUSP00000117040

UniGene: Mm.259072

Pathway Adipocytokine signaling pathway
AMPK signaling
Apelin signaling pathway
Glucagon signaling pathway
Insulin signaling pathway
Myocardial Fatty Acid Metabolism

Q&A and Customer Reviews

Q&A
Q:

Good day, I hope that this email finds you well.
For the ELISA kit CSB-EL018425MO the customer asks if the kit will also detect rat Peroxisome proliferator-activated receptor gamma coactivator 1-alpha. Have you tested the kit with rat samples? What is the homology of the immunogen(s) used to make the antibodies.
Would you have this information available to share?
Thank you for your assistance, it is greatly appreciated.

A:
Thanks for your inquiry!
For this target, we have rat kit available: CSB-EL018425RA. The raw material of rat kit is different from that of mouse kit. It is more suitable for rat samples. Sorry we didn't use mouse kit to test rat samples beforefo
Pls let me know if you have any further questions. Thank you.
Q:

Per customers request, please provide the following information regarding Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PPARGC1A ELISA Kit CSB-EL018425MO
a. Can you please confirm if lysis buffer is required for tissue homogenate?
b. Also, if this kit suitable for cell lysate samples?

A:
Thanks for your inquiry!
Generally, RIPA will contain components such as SDS, Triton X-100, and NP-40. Pls note:
1. It must not contain SDS (which will destroy the hydrogen bond of protein macromolecules causing protein denaturation);
2. For Triton X-100, NP 40 and other mild detergents, you should control the low concentration range (generally no more than 1%, but it not verified, so we are not clear about the specific impact);
3.EGTA and EDTA can be used as chelating agents for metal ions to remove metal ions, which has a great influence on BCA method, but we are not clear about the impact on elisa;
4 The sample should not contain NaN3 components, which will inhibit HRP activity and cause false negative.
Therefore, if the customer's sample has been prepared, you can do a pretest first but we are not clear about the final test result; if not prepared, it is recommended to follow the recommended method in our manual.
We suggest you do a pretest first if you want to test cell lysate samples.
Pls let me know if you have any further questions. Thank you.
Customer Reviews

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