Code | CSB-EL018425MO |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PPARGC1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 93 | ||||
Range % | 86-96 | |||||
1:2 | Average % | 104 | ||||
Range % | 97-108 | |||||
1:4 | Average % | 94 | ||||
Range % | 87-98 | |||||
1:8 | Average % | 99 | ||||
Range % | 92-102 |
The recovery of mouse PPARGC1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 87-97 | ||||
EDTA plasma (n=4) | 99 | 90-102 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Mouse PPARGC1A ELISA Kit was designed for the quantitative measurement of Mouse PPARGC1A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.
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For the ELISA kit CSB-EL018425MO the customer asks if the kit will also detect rat Peroxisome proliferator-activated receptor gamma coactivator 1-alpha. Have you tested the kit with rat samples? What is the homology of the immunogen(s) used to make the antibodies.
Would you have this information available to share?
Thank you for your assistance, it is greatly appreciated.
Per customers request, please provide the following information regarding Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PPARGC1A ELISA Kit CSB-EL018425MO
a. Can you please confirm if lysis buffer is required for tissue homogenate?
b. Also, if this kit suitable for cell lysate samples?