Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) ELISA kit

Instructions
Code CSB-EL018425MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Alternative Names Ppargc1a ELISA Kit; Pgc1 ELISA Kit; Pgc1a ELISA Kit; Ppargc1 ELISA Kit; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit; PGC-1-alpha ELISA Kit; PPAR-gamma coactivator 1-alpha ELISA Kit; PPARGC-1-alpha ELISA Kit
Abbreviation PPARGC1A
Uniprot No. O70343
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PPARGC1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %86-96
1:2Average %104
Range %97-108
1:4Average %94
Range %87-98
1:8Average %99
Range %92-102
Recovery
The recovery of mouse PPARGC1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-97
EDTA plasma (n=4)9990-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.730 2.582 2.656 2.466
7502.181 2.076 2.129 1.939
3751.365 1.345 1.355 1.165
187.50.871 0.832 0.852 0.662
940.550 0.541 0.546 0.356
470.385 0.378 0.382 0.192
23.50.288 0.279 0.284 0.094
00.192 0.188 0.190
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

 Q&A
Q:

Good day, I hope that this email finds you well.
For the ELISA kit CSB-EL018425MO the customer asks if the kit will also detect rat Peroxisome proliferator-activated receptor gamma coactivator 1-alpha. Have you tested the kit with rat samples? What is the homology of the immunogen(s) used to make the antibodies.
Would you have this information available to share?
Thank you for your assistance, it is greatly appreciated.

A:
Thanks for your inquiry!
For this target, we have rat kit available: CSB-EL018425RA. The raw material of rat kit is different from that of mouse kit. It is more suitable for rat samples. Sorry we didn't use mouse kit to test rat samples beforefo
Pls let me know if you have any further questions. Thank you.
Q:

Per customers request, please provide the following information regarding Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PPARGC1A ELISA Kit CSB-EL018425MO
a. Can you please confirm if lysis buffer is required for tissue homogenate?
b. Also, if this kit suitable for cell lysate samples?

A:
Thanks for your inquiry!
Generally, RIPA will contain components such as SDS, Triton X-100, and NP-40. Pls note:
1. It must not contain SDS (which will destroy the hydrogen bond of protein macromolecules causing protein denaturation);
2. For Triton X-100, NP 40 and other mild detergents, you should control the low concentration range (generally no more than 1%, but it not verified, so we are not clear about the specific impact);
3.EGTA and EDTA can be used as chelating agents for metal ions to remove metal ions, which has a great influence on BCA method, but we are not clear about the impact on elisa;
4 The sample should not contain NaN3 components, which will inhibit HRP activity and cause false negative.
Therefore, if the customer's sample has been prepared, you can do a pretest first but we are not clear about the final test result; if not prepared, it is recommended to follow the recommended method in our manual.
We suggest you do a pretest first if you want to test cell lysate samples.
Pls let me know if you have any further questions. Thank you.

Target Data

Function Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK. Isoform 4 specifically activates the expression of IGF1 and suppresses myostatin expression in skeletal muscle leading to muscle fiber hypertrophy.
Gene References into Functions
  1. The expression level of myocardial PGC-1alpha in MI mice with cardiac rupture after MI was significantly higher than that in the mice without cardiac rupture, and the ratio of myocardial NT-PGC-1alpha/PGC-1alpha was low. PMID: 30275441
  2. These findings show that melatonin attenuates the development of diabetes-induced cardiac dysfunction by preventing mitochondrial fission through SIRT1-PGC1alpha pathway, which negatively regulates the expression of Drp1 directly. PMID: 29575122
  3. Nicotinamide riboside (NR) can protect against ethanol induced liver injuries via replenishing NAD(+), reducing oxidative stress, and activating SirT1-PGC-1a-mitochondrial biosynthesis. Our data indicate that SirT1 plays an important role in the protection of NR against lipid accumulation and mitochondrial dysfunctions induced by ethanol PMID: 29679894
  4. Findings demonstrated that one bout of both uphill and downhill exercise trainings as well as 8 weeks of training could increase the expression of PGC-1alpha and FNDC5 genes in the muscle tissues and the UCP1 gene in the subcutaneous adipose tissue. PMID: 30218749
  5. Studied the involvement of peroxisome proliferative activated receptor gamma coactivator 1 alpha (PGC-1a) in beta-cell dysfunction and impairment of its ability to secrete insulin. PMID: 28259539
  6. At least in the subcutaneous fat of humans and mice, the levels of PGC-1a decrease during obesity, while its physical association with A20 increases. PMID: 29678181
  7. During catecholamine-stimulated lipolysis, Perilipin 5 is phosphorylated by protein kinase A and forms transcriptional complexes with PGC-1alpha and SIRT1 in the nucleus. Perilipin 5 promotes PGC-1alpha co-activator function by disinhibiting SIRT1 deacetylase activity. PMID: 27554864
  8. the coordinated expression of FNDC5 and PGC-1alpha may contribute to the increased levels of plasma irisin after exercise. PMID: 29415899
  9. Endogenous PGC1alpha expression exhibited protective effects against oxidative stress, glomerulosclerosis and tubulointerstitial fibrosis in experimental diabetic kidney disease. PMID: 29344670
  10. Resistin inhibited the activation of CREB, resulting in suppression of PGC-1alpha. Importantly, overexpressing PGC-1alpha can rescue the inhibitory effects of resistin on fatty acid beta oxidation. PMID: 29730652
  11. A cap-binding protein 80 (CBP80)-binding motif (CBM) in the C terminus of PGC-1alpha is critical for the association of PGC-1alpha with CBP80 at the 5' cap of target transcripts. PMID: 29654059
  12. findings support the hypothesis that maternal diabetes-induced ER stress increases CHOP expression which represses PGC-1alpha through suppressing the C/EBPbeta transcriptional activity, subsequently induces mitochondrial dysfunction and ultimately results in NTDs PMID: 28482072
  13. PGC-1alpha does not seem to regulate the activity of hepatic unfolded protein response in response to exercise training, but to influence the capacity of the liver to induce UPR in a pathway specific manner. PMID: 28801170
  14. AMPK-PGC-1a control of mitochondrial reactive oxygen species regulates Warburg metabolism. PMID: 28978464
  15. At 11 weeks, the MCK-PGC-1alpha animals had 50% greater glucose tolerance integrated area under the curve compared with WT. PMID: 28639297
  16. In the absence of serum, hypoxia and high glucose conditions, PGC-1alpha can regulate the expression of Bcl-2 and promote the survival of MSCs via PGC-1alpha/ERRalpha interaction. PMID: 28711948
  17. PGC-1alpha is involved in resveratrol-induced microglia M2 polarization. PGC-1alpha regulates microglia polarization through NF-kappaB, STAT6 and STAT3 pathways PMID: 28268115
  18. Study provides evidence that PGC-1a is a mediator of ischemic preconditioning that is downregulated in the steatotic liver, blunting the induction of protective antioxidant enzymes. PMID: 28269997
  19. that oxidatively-mediated reductions in parkin solubility and function in a mouse model of age-related sporadic PD coincides with increased PARIS levels and reduced PGC-1alpha signaling PMID: 27185595
  20. in the skeletal muscle, PGC-1alpha activates ALT2 gene expression, and alanine production may play roles in adaptation to fasting PMID: 29315328
  21. PPAR-beta increases PGC-1alpha content via a reduction in protein degradation. PMID: 28467933
  22. Muscle-specific PGC-1alpha transgenic mice have lower satellite cell number. SCs from these mice have a higher propensity for activation and proliferation. PMID: 27908291
  23. Full length-PGC-1alpha-deficient mice can be regarded not only as morphological but behavioral models of mitochondrial encephalopathies PMID: 27424777
  24. The Genomic Context and Corecruitment of SP1 Affect ERRalpha Coactivation by PGC-1alpha in Muscle Cells PMID: 27182621
  25. These findings indicate that IGF-II reduces PGC-1alpha expression in skeletal muscle cells through a mechanism involving PI3K-Akt-FoxO1 but not p38 MAPK or Erk1/2 MAPK pathways. PMID: 28374141
  26. Findings demonstrate that PGC-1alpha protects against podocyte depletion and phenotypic changes possibly by maintaining normal mitochondrial function. PMID: 26943584
  27. The involvement of mTOR-PGC-1alpha pathway in the connection between FTO and muscle differentiation is displayed. PMID: 28333151
  28. findings indicate that exercise intensity affected autophagy markers differently in skeletal muscle and suggest that PGC-1alpha regulates both acute and exercise training-induced autophagy in skeletal muscle potentially in a PGC-1alpha isoform specific manner PMID: 29049322
  29. EET-mediated increase in HO-1 levels require PGC-1alpha expression PMID: 27418542
  30. This change in the cellular location of p53 alleviates the abrogation of PGC-1a repression caused by nuclear p53, which may further increases PGC-1a expression PMID: 27497229
  31. Peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1alpha) exerts beneficial effects on muscle inflammation that might contribute to the therapeutic effects of elevated muscle PGC-1alpha in different models of muscle wasting. PMID: 27833743
  32. Adult conditional PGC-1alpha knock-out mice show a significant loss of dopaminergic neurons that is accompanied by a reduction of dopamine in the striatum. Study identifies also two brain-enriched isoforms of PGC-1alpha: PGC-1alpha1L (160 kDa) and PGC-1alpha1S (100 kDa). PMID: 27622213
  33. The study propose that gluconeogenesis driven by NTPGC- 1a, along with PGC-1a, contributes to the elevated blood glucose level in response to fasting. PMID: 27798359
  34. Metagenomic analysis revealed direct correlation between PPARGC1A, SNAI1, and metastatic lung disease. PMID: 28803067
  35. Destabilization of PGC1a is attributable to decreased p38 MAPK activation via diminished CaMKII signaling. Thus, we elucidate a pathway downstream of Ca(2+)-mediated CaMKII activation that is dysfunctional in C3KO(Capn3 knock-out mice ) mice, leading to reduced transcription of genes involved in muscle adaptation PMID: 27005420
  36. lncBATE10 can decoy Celf1 from Pgc1alpha, thereby protecting Pgc1alpha mRNA from repression by Celf1 PMID: 28763438
  37. this study shows that PGC-1alpha overexpression improves metabolism in virus-specific CD8+ T cells generated during acute infection PMID: 27496729
  38. this study reports that tumor-infiltrating T cells show a progressive loss of PGC1alpha, and that overexpression of PGC1alpha results in superior intratumoral T cell functions PMID: 27496732
  39. This is the first study to dissect the mechanism by which the antiadipogenic and anti-inflammatory lipid, Epoxyeicosatrienoic acid, induces the PGC-1alpha signaling cascade and reprograms the adipocyte phenotype by regulating mitochondrial function and HO-1 expression. PMID: 27224420
  40. The accelerated conversion of T4 to T3 within myocytes mediates part of the PGC-1a induction by treadmill exercise and its downstream effects on mitochondrial function PMID: 27302464
  41. Study demonstrates a novel role for peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) in nucleus accumbens (NAc) in cocaine action. PGC-1alpha is enhanced in NAc dopamine D1-medium spiny neurons, specifically after cocaine exposure. These data are consistent with increased active methylation and early growth response 3 binding at the PGC-1alpha promoter. PMID: 27939396
  42. These data reveal a new role for muscle PGC-1alpha in regulating the physiological adaptation to long-term LCHF diet administration. PMID: 28223292
  43. These results demonstrate a central role of skeletal muscle PGC-1alpha in the transcriptional regulation of systemic ketolytic capacity. PMID: 26849960
  44. PGC-1alpha expression in cardiomyocytes is regulated by interleukin-6 and is down-regulated in animals fed a high-fat diet. PMID: 27253588
  45. the data strengthen our previous findings that NT-PGC-1alpha regulates mitochondrial genes involved in thermogenesis and oxidative metabolism in brown and white adipocytes and further suggest that NT-PGC-1alpha regulates a broad spectrum of genes to meet cellular needs for adaptive thermogenesis. PMID: 27454177
  46. role of PGC-1alpha in renal mitochondrial biogenesis indicates that activation of PGC-1alpha in the context of renal disorders could be a valid therapeutic strategy to ameliorate renal mitochondrial dysfunction PMID: 27463191
  47. Results delineate a cross talk between a central pathway controlling metabolic regulation and cell adhesion, and identify PGC-1alpha as a molecular link between these two major cellular networks. PMID: 27984584
  48. Data suggest NT-Pgc-1alpha (alternative splicing isoform of nuclear protein Pgc-1alpha) is located in mitochondrial matrix in brown adipocytes, specifically enriched at D-loop region of mitochondrial DNA at promoter region of Lrpprc (leucine-rich PPR-motif containing protein); expression of NT-Pgc-1alpha (rather than Pgc-1alpha) in brown adipocytes appears to induce expression of electron transport chain complex proteins. PMID: 28473468
  49. Data suggest that PGC-1alpha and -1beta expression are not required for training-induced exercise performance, highlighting the contribution of PGC-1-independent mechanisms. PMID: 27780821
  50. Data show that resveratrol reduced mitochondrial reactive oxygen species (mROS) generation by promoting Sirt3 enrichment within the mitochondria and subsequent upregulation of FoxO3a-mediated mitochondria gene expression of PGC-1alpha and SOD2. PMID: 28286268

Show More

Hide All

Subcellular Location Nucleus, Nucleus, PML body
Tissue Specificity White quadriceps and red tibialis anterior (TA) muscles, liver, kidney and brown adipose tissue (at protein level). Skeletal muscle, brown adipose tissue, heart, kidney and brain.
Database Links

KEGG: mmu:19017

STRING: 10090.ENSMUSP00000117040

UniGene: Mm.259072

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1