Mouse Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) ELISA kit

Code CSB-EL009869MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse GPX4 ELISA Kit was designed for the quantitative measurement of Mouse GPX4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Abbreviation GPX4
Uniprot No. Q91XR9
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %86-95
1:2Average %100
Range %97-104
1:4Average %94
Range %85-97
1:8Average %98
Range %91-103
Recovery
The recovery of mouse GPX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9287-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.746 2.675 2.711 2.539
10002.218 2.189 2.204 2.032
5001.392 1.412 1.402 1.230
2500.854 0.834 0.844 0.672
1250.571 0.553 0.562 0.390
62.50.376 0.368 0.372 0.200
31.250.283 0.289 0.286 0.114
00.171 0.173 0.172
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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