Mouse Plasma protease C1 inhibitor(SERPING1) ELISA kit

Code CSB-EL021086MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
Alternative Names
Serping1 ELISA Kit; C1nh ELISA Kit; Plasma protease C1 inhibitor ELISA Kit; C1 Inh ELISA Kit; C1Inh ELISA Kit; C1 esterase inhibitor ELISA Kit; C1-inhibiting factor ELISA Kit; Serpin G1 ELISA Kit
Abbreviation
SERPING1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
5.6 ng/mL-360 ng/mL
Sensitivity
1.4 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Blood Coagulation
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPING1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 100  
Range % 95-105  
1:2000 Average % 104  
Range % 101-107  
1:4000 Average % 83  
Range % 80-86  
1:8000 Average % 98  
Range % 95-103  
Recovery
The recovery of mouse SERPING1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 85-92  
EDTA plasma (n=4) 96 93-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
360 2.559 2.569 2.564 2.461  
180 2.253 2.298 2.276 2.173  
90 1.822 1.843 1.833 1.730  
45 1.079 1.098 1.089 0.986  
22.5 0.602 0.634 0.618 0.515  
11.25 0.331 0.351 0.341 0.238  
5.6 0.205 0.220 0.213 0.110  
0 0.102 0.104 0.103    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse SERPING1 ELISA Kit was designed for the quantitative measurement of Mouse SERPING1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 5.6 ng/mL-360 ng/mL and the sensitivity is 1.4 ng/mL.

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Target Background

Function
(From Uniprot)
Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. May inhibit chymotrypsin and kallikrein.
Gene References into Functions
  1. C1INH prevented myocardial ischemia reperfusion injury in mice. PMID: 22705194
  2. Transgenic mice have been generated that express human C1 inhibitor mRNA and protein under the control of the human promoter and regulatory elements; C1nh production is demonstrated in mouse brain, spleen, liver, heart, kidney, and lung. PMID: 12421980
  3. In vivo, C1 inhibitor (C1INH) reduces the number of viable bacteria in the blood and peritoneal fluid and accelerates killing of bacteria by blood neutrophils and peritoneal macrophages. PMID: 17785834
  4. C1-inhibitor limits neointimal plaque formation and inflammation. This may involve blockade of complement activation, inhibition of leukocyte recruitment, and reduced triglyceride levels PMID: 18071075
  5. In addition to the protective activities mediated via inhibition of the complement system, these studies indicate that C1INH also plays a direct role in suppression of leukocyte transmigration into reperfused tissue. PMID: 18787060

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Subcellular Location
Secreted.
Protein Families
Serpin family
Database Links
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