Mouse Prokineticin-2(PROK2) ELISA kit

Code CSB-EL018747MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9QXU7
Lead Time 5-7 working days
Abbreviation PROK2
Protein Biological Process 1 Neurobiology
Target Name prokineticin 2
Alias BV8, KAL4, MIT1, PK2, protein Bv8 homolog
Species Mus musculus (Mouse)
Protein Biological Process 3 Biological rhythms
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.1 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PROK2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 90-95  
1:2 Average % 88  
Range % 84-95  
1:4 Average % 93  
Range % 88-97  
1:8 Average % 99  
Range % 94-102  
Recovery
The recovery of mouse PROK2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 91-96  
EDTA plasma (n=4) 95 91-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
800 2.866 2.842 2.854 2.758  
400 2.001 1.976 1.989 1.893  
200 1.129 1.132 1.131 1.035  
100 0.618 0.625 0.622 0.526  
50 0.344 0.358 0.351 0.255  
25 0.221 0.235 0.228 0.132  
12.5 0.152 0.144 0.148 0.052  
0 0.098 0.094 0.096    
References
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Function May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle (By similarity).
Subcellular Location Secreted
Protein Families AVIT (prokineticin) family
Tissue Specificity Expressed in the SCN and among a few other discrete brain areas, including the islands of Calleja, media l preoptic area of the hypothalamus and the shell of the nucleus accumbens. Highly expressed in testis. In the SCN, expression subjected to high ampli
Database Links

KEGG: mmu:50501

STRING: 10090.ENSMUSP00000032152

UniGene: Mm.87365

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