Mouse Protein Wnt-4(WNT4) ELISA kit

Code CSB-EL026137MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
wingless-type MMTV integration site family, member 4
Alternative Names
Wnt4 ELISA kit; Wnt-4Protein Wnt-4 ELISA kit
Abbreviation
WNT4
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Stem Cells
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 86    
Range % 82-91    
1:2 Average % 101    
Range % 98-105    
1:4 Average % 87    
Range % 84-91    
1:8 Average % 100    
Range % 95-105    
Recovery
The recovery of mouse WNT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 89 85-94    
EDTA plasma (n=4) 107 103-110    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
500 1.815 1.800 1.808 1.675    
250 1.211 1.301 1.256 1.123    
125 0.767 0.798 0.783 0.650    
62.5 0.485 0.468 0.477 0.344    
31.2 0.301 0.312 0.307 0.174    
15.6 0.218 0.209 0.214 0.081    
7.8 0.177 0.180 0.179 0.046    
0 0.132 0.134 0.133      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse WNT4 ELISA Kit was designed for the quantitative measurement of Mouse WNT4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

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Target Background

Function
(From Uniprot)
Ligand for members of the frizzled family of seven transmembrane receptors. Plays an important role in the embryonic development of the urogenital tract and the lung. Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development. Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries. Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary. Required for normal lung development and for normal patterning of trachael cartilage rings.
Gene References into Functions
  1. urinary Wnt4 could be a potential noninvasive biomarker for the early detection of tubular injury. PMID: 27600466
  2. GLP-1 promoted adipogenesis through the modulation of the Wnt4/beta-catenin signaling pathway PMID: 27504979
  3. Results suggest that the Wnt4 gene encodes signals that are important for various aspects of female reproductive tract development. PMID: 26721931
  4. Our functional study revealed that WNT4 molecules were involved in regulating zygotic cleavage and early embryogenesis PMID: 26700598
  5. Data indicate that a balance between supporting cell self-renewal and differentiation is maintained in the developing ovary by relative Wnt4 protein/beta-catenin and p27Kip1 protein (p27)/Forkhead box L2 (FOXL2) activities. PMID: 26939755
  6. decreased expression of Wnt4 in the aging thymus may be one of the molecular triggers underlying the process of age-related thymic senescence. PMID: 26397044
  7. Mir-29c regulates WNT4 signaling. PMID: 26484393
  8. Wnt4 is essential to normal mammalian lung development. PMID: 26321050
  9. RSPO1, WNT4, and beta-catenin have roles in the signaling pathway during ovarian differentiation in mice. PMID: 25187620
  10. The Runx-1 gene can be a Wnt-4 signalling target, and that Runx-1 and Wnt-4 are mutually interdependent in their expression. PMID: 25645944
  11. Progesterone and Wnt4 control stem cell function through a luminal-myoepithelial crosstalk with Wnt4 acting independent of progesterone receptor perinatally. PMID: 25603931
  12. significant role in supporting thymocyte development and maturation PMID: 24768153
  13. results suggest that Wnt4 signaling could be an attractive therapeutic target for treating osteoporosis and preventing skeletal aging PMID: 25108526
  14. Data indicate that Wnt4 signaling is necessary during maturation of the ovarian follicle. PMID: 24371124
  15. Data from several types of in vitro angiogenesis assays suggest that Wnt4 (but not Wnt1) is a potent inhibitor of capillary outgrowth; Wnt4 and Wnt1 appear to be secreted by vascular endothelial cells into extracellular matrix during angiogenesis. PMID: 22607657
  16. Wnt4-null and Rspo1-null pregranulosa cells transition through a differentiated granulosa cell state prior to transdifferentiating towards a Sertoli cell fate. PMID: 24036309
  17. Wnt4 signaling through beta-catenin stabilization is sufficient to drive spontaneous myofibroblast differentiation in interstitial pericytes and fibroblasts, emphasizing the importance of this pathway in renal fibrosis. PMID: 23766539
  18. Endocardial to myocardial notch1-wnt4-bmp2 axis regulates early heart valve development. PMID: 23560082
  19. this study identifies Rspo1 and Wnt4 as two new regulators of cell proliferation in the early gonad regardless of its sex, in addition to the specific role of these genes in ovarian differentiation. PMID: 23095882
  20. The repression of many female genes including Wnt4 is necessary for the bipotential gonad to adopt the male testicular fate. PMID: 22705479
  21. Our analyses show that Wt1, Sf1, and Wnt4 can be dosage-sensitive in mice, depending on the genetic background, and provide insights into the mechanisms of B6 XY sex reversal PMID: 22496664
  22. WNT4 was found to signal via the CTNNB1 pathway in Sertoli cells. PMID: 22253774
  23. These data reveal a new role for Wnt4 in mammalian Neuromuscular junction formation. PMID: 22253844
  24. Wnt4 regulates steady-state thymic cellularity by a thymic epithelial cell (TEC)-dependent mechanism. The absence of Wnt4 suppressed fetal and postnatal thymic expansion and resulted in decreased TEC numbers. PMID: 21937690
  25. Wnt-4 modulates canonical Wnt signaling and acts as regulator of beta-cell proliferation and inflammatory cytokine release. PMID: 21771967
  26. An important role of Wnt-4 in thymic senescence, is reported. PMID: 21453014
  27. Activation of canonical Wnt signaling was evident as indicated by increased Wnt4 activation in livers of hepatocellular carcinoma farnesoid X-knockout mice. PMID: 21430080
  28. These results establish a functional role for non-canonical Wnt signaling in hematopoiesis. PMID: 21541287
  29. Wnt4 activated the canonical beta-catenin pathway during myogenic differentiation in both myoblasts and satellite cells and negatively regulated myostatin's expression and its regulating pathways. PMID: 21248078
  30. Maintenance of female germ cells hinge upon a delicate balance between positive (WNT4 and beta-catenin) and negative (activin betaB) regulators derived from the somatic cells in the fetal ovary. PMID: 20454446
  31. WNT4 is a critical regulator not only of proper postnatal uterine development, but also embryo implantation and decidualization. PMID: 21163860
  32. Wnt/beta-catenin signaling occurs in proliferating vascular smooth muscle cells during intimal thickening. This is a result of Wnt4 upregulation. PMID: 21193738
  33. Wnt-4 deficiency allows 20% of the germ cells to initiate meiosis in the ovary, whereas meiosis is inhibited completely in the Wnt-4/Wnt-5a double mutant. PMID: 20106871
  34. WNT4 is required for normal antral follicle development and may act by regulating granulosa cell functions including steroidogenesis. PMID: 20371632
  35. Notch2 overexpression leads to ectopic Wnt4 expression and premature tubule formation. PMID: 20299358
  36. data indicate that Wnt4 levels must be regulated in chondrocytes for normal growth plate development and skeletogenesis PMID: 17505543
  37. The current results support a view that WNT4 may have a role in oocyte selection and follicle formation and maturation in human ovaries. PMID: 19962424
  38. Wnt4(EGFPCre)-mediated inactivation of Wnt4 function considerably reduced the amount of Wnt4 transcripts, led to a severe defect in kidney development, and caused the female embryos to undergo partial sex reversal to males PMID: 19830824
  39. role for Wnt-4 in the pathogenesis of renal fibrosis. PMID: 11832423
  40. wnt-4 is expressed at distinct stages of follicular development. PMID: 11861511
  41. results point to a role for Wnt-4 in adrenal gland development and function PMID: 12399432
  42. Wnt4 signaling mediates the increased expression of Dax-1 as the ovary becomes sexually differentiated PMID: 12554773
  43. Wnt-4 dependent signaling cascades regulate steroidogenic factor-1-dependent transcription of genes in adrenal cortex PMID: 12732619
  44. Data show that the role of WNT4 in gonad development is to pattern the sex-specific vasculature and to regulate steroidogenic cell recruitment. PMID: 12835383
  45. Wnt4 mRNA is expressed in a decreasing anterior-to-posterior gradient in the floor plate; commissural axons lacking the Wnt receptor Frizzled3 displayed anterior-posterior guidance defects after midline crossing PMID: 14671310
  46. matrilysin may have a role in renal tubular injury and progression of tubulointerstitial fibrosis, and Wnt4 may regulate matrilysin expression in the kidney PMID: 15149334
  47. germ cells in the ovarian cortex are almost completely lost in both Wnt4 and follistatin null gonads before birth PMID: 15162500
  48. Wnt-4 binds both canonical and noncanonical Frizzled receptors, thereby activating Wnt signaling pathways that may each contribute to kidney tubulogenesis. PMID: 15265686
  49. Results identify WNT4 as a new factor involved in the mammalian testis determination pathway and show that genes can have a specific but distinct role in both male and female gonad development. PMID: 15581876
  50. Wnt-4 signaling normally acts to suppress testosterone biosynthesis in the female, and that testosterone is the putative mediator of the masculinization phenotype in Wnt-4-deficient females PMID: 15932923

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Wnt family
Tissue Specificity
In adults in lung and brain.
Database Links
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