Mouse Protein-arginine deiminase type-4(PADI4) ELISA kit

Product Details

Customer Reviews and Q&A

Target Background

Product Details

Target Name

peptidyl arginine deiminase, type IV

Alternative Names

Padi4 ELISA Kit; Pad4 ELISA Kit; Pdi4 ELISA Kit; Protein-arginine deiminase type-4 ELISA Kit; EC 3.5.3.15 ELISA Kit; Peptidylarginine deiminase IV ELISA Kit; Protein-arginine deiminase type IV ELISA Kit

Abbreviation

PADI4

Uniprot No.

Q9Z183

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

4.7 pg/mL-300 pg/mL

Sensitivity

1.17 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PADI4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-99
1:2	Average %	95
	Range %	89-101
1:4	Average %	96
	Range %	92-110
1:8	Average %	93
	Range %	86-102

Recovery

The recovery of mouse PADI4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	88-105
EDTA plasma (n=4)	96	89-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.228	2.230	2.229	2.051
150	1.457	1.378	1.418	1.240
75	0.854	0.822	0.838	0.660
37.5	0.483	0.513	0.498	0.320
18.75	0.355	0.363	0.359	0.181
9.4	0.274	0.259	0.267	0.089
4.7	0.233	0.226	0.230	0.052
0	0.175	0.181	0.178

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse PADI4 ELISA Kit was designed for the quantitative measurement of Mouse PADI4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.17 pg/mL.

Target Background

Function
(From Uniprot)

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.

Gene References into Functions

PAD4 in neutrophils is essential for kidney NET formation and ischemic kidney injury. PMID: 29061334
PADi4 is critical for neutrophil extracellular traps formation, but is dispensable for generalized citrullination PMID: 28188029
sFlt-1 overexpression in Padi4(-/-) mice resulted in dramatically lower inflammatory and thrombotic response, which was accompanied by significant reduction in pregnancy losses. Inhibition of NETosis may serve as a novel target in disorders of impaired placentation. PMID: 28007693
a role for NETs in cardiac fibrosis and conclude that PAD4 regulates age-related organ fibrosis and dysfunction. PMID: 28031479
PADI4 exacerbates arthritis with diverse immunological modifications. PMID: 26293116
Selective inhibition of peptidylarginine deiminase IV with Cl-amidine blocked NET formation, reduced atherosclerotic lesion area, and delayed time to carotid artery thrombosis in a photochemical injury model. PMID: 24425713
Padi4 is part of the pluripotency transcriptional network, binding to regulatory elements of key stem-cell genes and activating their expression PMID: 24463520
PAD4-mediated chromatin decondensation in the neutrophil is crucial for pathological venous thrombosis and present neutrophil activation and PAD4 as potential drug targets for deep vein thrombosis. PMID: 23650392
PAD4 is dispensable in this effector phase model of arthritis PMID: 22551352
analysis of regulation of histone modification and chromatin structure by the p53-PADI4 pathway PMID: 22334079

Hide All

Subcellular Location

Cytoplasm. Nucleus. Cytoplasmic granule.

Protein Families

Protein arginine deiminase family

Tissue Specificity

Expressed in pluripotent embryonic stem and induced pluripotent stem cells but not multipotent neural stem cells.

Database Links

KEGG: mmu:18602

STRING: 10090.ENSMUSP00000026381

UniGene: Mm.250358

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-EL017379MO
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.