Mouse Protein disulfide-isomerase A4(PDIA4) ELISA kit

Code CSB-EL017722MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
protein disulfide isomerase family A, member 4
Alternative Names
Pdia4 ELISA Kit; Cai ELISA Kit; Erp72Protein disulfide-isomerase A4 ELISA Kit; EC 5.3.4.1 ELISA Kit; Endoplasmic reticulum resident protein 72 ELISA Kit; ER protein 72 ELISA Kit; ERp-72 ELISA Kit; ERp72 ELISA Kit
Abbreviation
PDIA4
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDIA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %81-92
1:2Average %100
Range %93-104
1:4Average %90
Range %85-95
1:8Average %99
Range %91-103
Recovery
The recovery of mouse PDIA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9285-96
EDTA plasma (n=4)9589-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.654 2.543 2.599 2.417
102.135 2.077 2.106 1.924
51.345 1.332 1.339 1.157
2.50.824 0.843 0.834 0.652
1.250.526 0.545 0.536 0.354
0.6250.359 0.369 0.364 0.182
0.3120.278 0.266 0.272 0.090
00.184 0.180 0.182
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse PDIA4 ELISA Kit was designed for the quantitative measurement of Mouse PDIA4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Gene References into Functions
  1. activated by IL11 in placenta PMID: 28487027
  2. PDIA4 as a negative regulator of cancer cell apoptosis. PMID: 28534513
  3. ERp72 functions separately from protein disulfide isomerase and ERp57 in supporting platelet aggregation. Defective aggregation of ERp72-null platelets was recovered by ERp72, but not other thiol isomerases. PMID: 28576878
  4. A network of endoplasmic reticulum ERp57, ERp72, and ERp29 facilitate polyomavirus infection. PMID: 21159867
  5. There were increased numbers of ERp72-immunoreactive cells in the dorsal and lateral cortex during sleep deprivation. PMID: 12535952
  6. These results suggest that domains 1 and 2 of ERp72 contribute to catalytic efficacy, and domain 3 to binding affinity. PMID: 16333982

Show More

Hide All

Subcellular Location
Endoplasmic reticulum lumen. Melanosome.
Protein Families
Protein disulfide isomerase family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1