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Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

Protocol
Code CSB-E08685m
Size 96T,5×96T,10×96T
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Product Details

Target Name
surfactant protein A1
Alternative Names
Sftpa1 ELISA Kit; Sftp-1 ELISA Kit; Sftp1 ELISA Kit; Sftpa ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
Abbreviation
SFTPA1
Uniprot No.
P35242
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1000Average %84
Range %80-92
1:2000Average %96
Range %91-105
1:4000Average %98
Range %93-103
1:8000Average %93
Range %86-98
Recovery
The recovery of mouse SP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-95
EDTA plasma (n=4)9390-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.637 2.714 2.676 2.549
1002.331 2.368 2.350 2.223
501.847 1.901 1.874 1.747
251.273 1.267 1.270 1.143
12.50.845 0.869 0.857 0.730
6.250.542 0.583 0.563 0.436
3.120.322 0.317 0.320 0.193
00.128 0.126 0.127 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse SFTPA1 ELISA Kit was designed for the quantitative measurement of Mouse SFTPA1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

Related Products

Sftpa1 Proteins

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Target Background

Function
(From Uniprot)
In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
Protein Families
SFTPA family
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