Target Name
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Alternative Names
Sema3d ELISA Kit; Semaphorin-3D ELISA Kit
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
84-95 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-98 |
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| 1:4 |
Average % |
99 |
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| Range % |
96-102 |
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| 1:8 |
Average % |
96 |
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| Range % |
89-103 |
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Recovery
| The recovery of mouse SEMA3D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
86 |
80-94 |
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| EDTA plasma (n=4) |
98 |
92-104 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1500 |
2.433 |
2.332 |
2.383 |
2.224 |
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| 750 |
2.049 |
1.928 |
1.989 |
1.830 |
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| 375 |
1.339 |
1.318 |
1.329 |
1.170 |
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| 187.5 |
0.727 |
0.741 |
0.734 |
0.575 |
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| 94 |
0.485 |
0.496 |
0.491 |
0.332 |
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| 47 |
0.280 |
0.291 |
0.286 |
0.127 |
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| 23.5 |
0.221 |
0.225 |
0.223 |
0.064 |
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| 0 |
0.159 |
0.158 |
0.159 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Mouse SEMA3D ELISA Kit was designed for the quantitative measurement of Mouse SEMA3D protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL .
