Recombinant Mouse Semaphorin-3D (Sema3d)

Code CSB-YP806831MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP806831MO
MSDS
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Source E.coli
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Code CSB-EP806831MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP806831MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP806831MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Sema3d
Uniprot No.
Alternative Names
Sema3d; Semaphorin-3D
Species
Mus musculus (Mouse)
Expression Region
38-777
Target Protein Sequence
SKQ NIPRLKLTYK DLLLSNTCIP FLGSSEGLDF QTLLLDEERG ILLLGAKDHV FLLSLVDLNK NFKKIYWPAA KERVELCKLA GKDANAECAN FIRVLQPYNK THVYVCGTGA FHPLCGYIDL GANKEELIFK LDTHNLESGR LKCPFDPQQP FASVMTDEHL YSGTASDFLG KDTAFTRSLG LMQDHHSIRT DISEHHWLNG AKFIGTFPIP DTYNPDDDKI YFFFRESSQE GSTSDRSILS RVGRVCKNDV GGQRSLINKW TTFLKARLIC SIPGSDGADT HFDELQDIYL LPTRDERNPV VYGVFTTTSS IFKGSAVCVY SMADIRAVFN GPYAHKESAD HRWVQYDGRI PYPRPGTCPS KTYDPLIKST RDFPDDVISF IRRHPVMYKS VYPVAGAPTF KRINVDYRLT QIVVDHVVAE DGQYDVMFLG TDIGTVLKVV SISKEKWNME EVVLEELQVF KHPTAILNME LSLKQQQLYV GSWDGLVQLS LHRCDTYGKA CADCCLARDP YCAWDGNACS RYAPTSKRRA RRQDVKYGDP ITQCWDIEDS ISHETADEKV IFGIEFNSTF LECIPKSQQA SVEWYIQRSG DEHREELKPD ERIIKTDYGL LIRSLQKKDS GMYYCKAQEH TFIHTIVKLT LNVIENEQME NTQRAEYQEG QVKDLLAESR LRYKDYIQIL SSPNFSLDQY CEQMWYKEKR RQRNKGSPKW KHMQEMKKKR NRRHHRDLDE LQRSVAT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin.
Gene References into Functions
  1. Authors did not find evidence for genetic interaction between Ret and Sema3d affecting survival, presence of myenteric plexus or intestine transcriptome. PMID: 28334784
  2. Data indicate that annexin A2 (AnxA2) regulates the secretion of semaphorin 3D (Sema3D) from pancreatic ductal adenocarcinoma (PDA) cells. PMID: 26243191
  3. Semaphorin 3d requires neuropilin 1 or PI3K/Akt, whereas semaphorin 3e requires plexin D1 in directing endothelial motility. PMID: 24825896
  4. In the absence of Sema3d, endothelial tubes form in a region that is normally avascular PMID: 23685842
  5. Two proepicardial markers (Scleraxis and Semaphorin3D) were identified that genetically delineate heretofore uncharacterized proepicardial subcompartments. PMID: 22421048
  6. Sema 3D and Sema 3F genes are overlappingly and distinctly expressed in the developing parathyroid and thymus, respectively, and they might play roles in the interactions between these organs and surrounding tissues PMID: 18489001

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Subcellular Location
Secreted.
Protein Families
Semaphorin family
Database Links
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