Target Name
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
Alternative Names
Sema3f ELISA Kit; Semaphorin-3F ELISA Kit; Semaphorin IV ELISA Kit; Sema IV ELISA Kit
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | | |
Inter-assay Precision (Precision between assays): CV%<10% | | | | |
Three samples of known concentration were tested in twenty assays to assess. | | | |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| Sample | Serum(n=4) | | |
1:1 | Average % | 97 | | |
Range % | 92-103 | | |
1:2 | Average % | 91 | | |
Range % | 87-97 | | |
1:4 | Average % | 92 | | |
Range % | 88-96 | | |
1:8 | Average % | 101 | | |
Range % | 96-106 | | |
Recovery
The recovery of mouse SEMA3F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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Sample Type | Average % Recovery | Range | | |
Serum (n=5) | 91 | 88-94 | | |
EDTA plasma (n=4) | 107 | 103-111 | | |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.567 | 2.699 | 2.633 | 2.529 | | | 10 | 2.144 | 2.244 | 2.194 | 2.090 | | | 5 | 1.569 | 1.625 | 1.597 | 1.493 | | | 2.5 | 1.025 | 0.989 | 1.007 | 0.903 | | | 1.25 | 0.748 | 0.746 | 0.747 | 0.643 | | | 0.625 | 0.462 | 0.454 | 0.458 | 0.354 | | | 0.312 | 0.210 | 0.223 | 0.217 | 0.113 | | | 0 | 0.103 | 0.105 | 0.104 | | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Mouse SEMA3F ELISA Kit was designed for the quantitative measurement of Mouse SEMA3F protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.