Mouse Semaphorin-3F(SEMA3F) ELISA kit

Code CSB-EL020985MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
Alternative Names
Sema3f ELISA Kit; Semaphorin-3F ELISA Kit; Semaphorin IV ELISA Kit; Sema IV ELISA Kit
Abbreviation
SEMA3F
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 92-103
1:2 Average % 91
Range % 87-97
1:4 Average % 92
Range % 88-96
1:8 Average % 101
Range % 96-106
Recovery
The recovery of mouse SEMA3F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 88-94
EDTA plasma (n=4) 107 103-111
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.567 2.699 2.633 2.529
10 2.144 2.244 2.194 2.090
5 1.569 1.625 1.597 1.493
2.5 1.025 0.989 1.007 0.903
1.25 0.748 0.746 0.747 0.643
0.625 0.462 0.454 0.458 0.354
0.312 0.210 0.223 0.217 0.113
0 0.103 0.105 0.104  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse SEMA3F ELISA Kit was designed for the quantitative measurement of Mouse SEMA3F protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

Gene References into Functions
  1. data suggest that Sema3f and Nrp2 are dispensable for the exclusion of vessels from the cap mesenchyme during kidney development. PMID: 28929539
  2. Sema3f is suppressed in outer nuclear layer in very low-density lipid-receptor deficient retina. PMID: 28373097
  3. Sema3F functions as an unconventional Sema3 that promotes extraembryonic angiogenesis by inhibiting the Myc-regulated synthesis of Thbs1 in visceral yolk sac epithelial cells. PMID: 28729362
  4. Nrp2 deficiency increased blood vessel permeability and decreased lymphatic vessel drainage during inflammation, highlighting the importance of the NRP2/SEMA3F pathway in the modulation of tissue swelling and resolution of postinflammatory edema. PMID: 27751443
  5. Sema3F plays important roles in the development of neuronal circuitry underlying the regulation of some aspects of anxiety and fear responses. PMID: 26856818
  6. The authors demonstrate that expression of Semaphorin-3F in the outer hair cell region inhibits type I spiral ganglion neuron process extension by activating Neuropilin-2 receptors expressed on spiral ganglion neurons. PMID: 26302206
  7. the embryonically developed wiring deficits in Sema3F mutants persist until adulthood PMID: 25874621
  8. Sema4A promotes Th1 and Th17 differentiation and increases adhesive activation of T cells to endothelial cells, even in the presence of IFN-beta. PMID: 24439904
  9. Sema3F is secreted by early-arriving axons of olfactory sensory neurons and is deposited at the anterodorsal olfactory bulb to repel Nrp2-positive axons that arrive later. PMID: 20550939
  10. These data suggest that Sema3F plays a relatively restricted, if any, role in its receptor expression and postnatal development of the somatosensory cortex, the cerebellum, and the main olfactory system. PMID: 20006658
  11. Sema3F is the principal ligand for Npn-2-mediated axon guidance events in vivo and is a critical determinant of limbic and peripheral nervous system circuitry PMID: 12890759
  12. Sema3F alternatively spliced isoforms share common axonal chemorepulsive properties and binding to neuropilin receptors, and are found to regulate endothelial cell morphology by remodeling lamellipodial protrusions. PMID: 14572462
  13. We describe a related signaling pathway involving secreted class 3 semaphorins, neuropilins, and a plexin receptor, PlexinD1, expressed by endothelial cells PMID: 15239958
  14. Using sema3F-/- mice, we show that Sema3F is critical for vomeronasal sensory neuron axonal fasciculation and for segregation of these sensory afferents from the main olfactory system and lamina-specific targeting of olfactory sensory axons PMID: 15483127
  15. Npn2/Sema3f signaling guides neural crest migration through the somite. PMID: 16319111
  16. Genetic evidence for a Nrp2-Sema3f interaction as a determinant of the wiring of axons of gucy2d+ neurons into the unusual configuration of necklace glomeruli. PMID: 17942483
  17. An important regulator of the ventral tangential cell migration stream, confining the migrating neurons on the telencephalon surface by repelling from the deeper domain. PMID: 18434520
  18. Sema 3D and Sema 3F genes are overlappingly and distinctly expressed in the developing parathyroid and thymus, respectively, and they might play roles in the interactions between these organs and surrounding tissues PMID: 18489001
  19. Semaphorin3F induces significant growth cone collapse of temporal, but not nasal, RGCs expressing high levels of Neuropilin-2. PMID: 18942144
  20. Data demonstrate that methyl-CpG binding protein 2 deficiency disrupts the establishment of neural connections before synaptogenesis by altering semaphorin 3F function. PMID: 19628041
  21. Soluble neuropilin-2Fc did not inhibit repulsion but increased the repellent effect of semaphorin 3F. PMID: 19790074

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Subcellular Location
Secreted.
Protein Families
Semaphorin family
Tissue Specificity
Expressed ubiquitously in adulthood. During embryogenesis, expressed in subregions of the central nervous system and various other tissues like skin, kidney, lung and intestine.
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