Mouse Thromboxane B2,TXB2 ELISA Kit

Code CSB-E08048m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Thromboxane B2,TXB2
Abbreviation
TXB2
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
2.35 pg/mL-150 pg/mL
Sensitivity
0.58 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 99  
Range % 95-104  
1:2 Average % 89  
Range % 84-94  
1:4 Average % 90  
Range % 85-96  
1:8 Average % 98  
Range % 93-103  
Recovery
The recovery of mouse TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 94-102  
EDTA plasma (n=4) 91 86-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
150 2.238 2.112 2.175 2.006  
75 1.654 1.695 1.675 1.506  
37.5 1.048 1.112 1.080 0.911  
18.75 0.568 0.604 0.586 0.417  
9.4 0.314 0.338 0.326 0.157  
4.7 0.229 0.237 0.233 0.064  
2.35 0.204 0.216 0.210 0.041  
0 0.171 0.166 0.169    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse TXB2 ELISA Kit was designed for the quantitative measurement of Mouse TXB2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 2.35 pg/mL-150 pg/mL and the sensitivity is 0.58 pg/mL .

Customer Reviews and Q&A

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 Q&A
Q:

1) Does the kit contain any human derived material? If so, please describe
what it is.
2) Does the kit contain any animal derived material? If so please describe
what it is. Please let us know if it contains polyclonal or monoclonal
antibodies and what species the antibodies are from?

A:
Thanks for your inquiry.
1)The kit doesn't contain any human derived material;
2)Some components in the kit contain commercial BSA(it is purified).
Capture antibody is rat monoclonal antibody and detection antibody is goat polyclonal antibody.
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