Mouse Thromboxane B2,TXB2 ELISA Kit

Instructions
Code CSB-E08048m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Thromboxane B2,TXB2
Abbreviation TXB2
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 2.35 pg/mL-150 pg/mL
Sensitivity 0.58 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 99  
Range % 95-104  
1:2 Average % 89  
Range % 84-94  
1:4 Average % 90  
Range % 85-96  
1:8 Average % 98  
Range % 93-103  
Recovery
The recovery of mouse TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 94-102  
EDTA plasma (n=4) 91 86-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
150 2.238 2.112 2.175 2.006  
75 1.654 1.695 1.675 1.506  
37.5 1.048 1.112 1.080 0.911  
18.75 0.568 0.604 0.586 0.417  
9.4 0.314 0.338 0.326 0.157  
4.7 0.229 0.237 0.233 0.064  
2.35 0.204 0.216 0.210 0.041  
0 0.171 0.166 0.169    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

1) Does the kit contain any human derived material? If so, please describe
what it is.
2) Does the kit contain any animal derived material? If so please describe
what it is. Please let us know if it contains polyclonal or monoclonal
antibodies and what species the antibodies are from?

A:
Thanks for your inquiry.
1)The kit doesn't contain any human derived material;
2)Some components in the kit contain commercial BSA(it is purified).
Capture antibody is rat monoclonal antibody and detection antibody is goat polyclonal antibody.

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