Rat Thromboxane B2,TXB2 ELISA Kit

Code CSB-E08047r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Thromboxane B2,TXB2
Abbreviation
TXB2
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.9 pg/mL-250 pg/mL
Sensitivity
0.97 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 88-98  
1:2 Average % 95  
Range % 91-102  
1:4 Average % 99  
Range % 94-106  
1:8 Average % 93  
Range % 87-97  
Recovery
The recovery of rat TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 90-103  
EDTA plasma (n=4) 92 88-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
250 1.990 1.890 1.940 1.785  
125 1.298 1.198 1.248 1.093  
62.5 0.786 0.766 0.776 0.621  
31.25 0.545 0.525 0.535 0.380  
15.6 0.341 0.321 0.331 0.176  
7.8 0.292 0.272 0.282 0.127  
3.9 0.215 0.211 0.213 0.058  
0 0.156 0.154 0.155    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This rat TXB2 ELISA Kit is suitable for qualitatively determining rat concentrations in serum, plasma, or tissue homogenates in vitro. TXB2, an inactive metabolite/product of thromboxane A2, is a potent vasoconstrictor and platelet aggregating agent. It is important in the platelet-release reaction and induces irreversible platelet aggregation. TXB2 has been measured in a variety of clinical entities, particularly in coronary artery disease, where plasma elevations have been reported in coronary vasospasm.

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of rat TXB2 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-rat TXB2 antibody. Biotin-labeled TXB2 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the TXB2 in the rat samples. TXB2 binds to the biotinylated anti-TXB2 rat monoclonal antibody. And the biotin on the biotinylated anti-TXB2 rat monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of TXB2 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

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