Rat Thromboxane B2,TXB2 ELISA Kit

Code CSB-E08047r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Thromboxane B2,TXB2
Abbreviation TXB2
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.9 pg/mL-250 pg/mL
Sensitivity 0.97 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 88-98  
1:2 Average % 95  
Range % 91-102  
1:4 Average % 99  
Range % 94-106  
1:8 Average % 93  
Range % 87-97  
The recovery of rat TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 90-103  
EDTA plasma (n=4) 92 88-98  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
250 1.990 1.890 1.940 1.785  
125 1.298 1.198 1.248 1.093  
62.5 0.786 0.766 0.776 0.621  
31.25 0.545 0.525 0.535 0.380  
15.6 0.341 0.321 0.331 0.176  
7.8 0.292 0.272 0.282 0.127  
3.9 0.215 0.211 0.213 0.058  
0 0.156 0.154 0.155    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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