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- An assay plate --- The 96-well plate has been pre-coated with an anti-mouse T3 antibody.
- Standard (6 x 1 ml) --- Reconstitute one of the standards with sample diluent at dilution series and draw the standard curve.
- Biotin-conjugated T3 (1 x 6 ml)
- HRP-avidin (1 x 6 ml)--- HRP catalyzes the TMB substrate for color development and needs to be diluted before use.
- Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away the unbound solution and non-specific substances.
- Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
- Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
- Stop Solution (1 x 7 ml) --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
- Four Adhesive Strip (For 96 wells) --- Cover the microtiter plate when incubation.
- An instruction manual
