Sheep tri-iodothyronine (T3) ELISA kit

Code CSB-EQ027510SH
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
tri-iodothyronine (T3)
Abbreviation
T3
Species
Ovis aries (Sheep)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.5 ng/mL-8 ng/mL
Sensitivity
0.5 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheepT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 90-100
1:2 Average % 87
Range % 85-96
1:4 Average % 95
Range % 90-100
1:8 Average % 95
Range % 82-99
Recovery
The recovery of sheep T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 85-102
EDTA plasma (n=4) 96 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average  
8 0.172 0.165 0.169  
4 0.289 0.278 0.284  
2 0.549 0.557 0.553  
1 1.252 1.263 1.258  
0.5 2.124 2.141 2.133  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Sheep tri-iodothyronine (T3) ELISA kit is a cutting-edge tool for detecting T3 in a variety of samples, including serum, plasma, cell culture supernates, and tissue homogenates. This ELISA kit is specifically designed for Ovis aries (Sheep) and offers high accuracy, sensitivity, and reliability.

This kit has a detection range of 0.5 ng/mL-8 ng/mL, with a sensitivity of 0.5 ng/mL. It provides a quick and convenient assay time of 1-5 hours, with a sample volume of 50-100ul. The detection wavelength is 450 nm. All these makes it ideal for signal transduction and thyroid function research applications.

This ELISA kit uses a sandwich method of measurement, ensuring accurate and precise detection of T3. It offers unparalleled accuracy, sensitivity, and convenience, making it an essential tool for researchers and scientists studying signal transduction in Ovis aries.

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