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Goat Tri-iodothyronine(T3) ELISA KIT

Goat Tri-iodothyronine(T3) ELISA KIT

Protocol

Code	CSB-E13385G
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

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Product Details

Target Name

Tri-iodothyronine(T3)

Abbreviation

Species

Capra hircus (Goat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.5 ng/mL-8 ng/mL

Sensitivity

0.5 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of goat T3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-103
1:2	Average %	95
	Range %	85-102
1:4	Average %	93
	Range %	90-97
1:8	Average %	94
	Range %	88-99

Recovery

The recovery of goat T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	86-102
EDTA plasma (n=4)	96	92-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
8	0.166	0.168	0.167
4	0.281	0.288	0.285
2	0.558	0.553	0.556
1	1.262	1.247	1.255
0.5	2.105	2.138	2.122

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Goat Tri-iodothyronine (T3) ELISA Kit was designed for the quantitative measurement of Goat Tri-iodothyronine (T3) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.5 ng/mL-8 ng/mL and the sensitivity is 0.5 ng/mL.

Customer Reviews and Q&A

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■ Q&A

I'm studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

Thanks for your inquiry. We have the mentioned kit available.For example:
#CSB-E13385G,Goat Tri-iodothyronine(T3) ELISA KIT *96T (If duplicate done,22 kits is advised;If not,only 11 kits is ok)
the standard wells are S1-S5 and duplicate is advised.With a blank well,the number of standard wells is 5×2+1=11.The rest is for samples wells,85=96-11(If duplicate is done for sample,then the number will be the half of that.)
If you want to detect diary goat samples,we suggest you do pre-testing first.

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Goat Tri-iodothyronine(T3) ELISA KIT

Product Details

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Customer Reviews and Q&A