Mouse advanced glycation end products,AGEs ELISA Kit

Mouse advanced glycation end products,AGEs ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Mouse advanced glycation end products,AGEs ELISA Kit

Name: Mouse advanced glycation end products,AGEs ELISA Kit
Brand: CUSABIO
SKU: CSB-E09414m

Citations (10) Protocol MSDS

Code	CSB-E09414m
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Product Details
Citations (10)
Related Products
Customer Reviews and Q&A

Target Name

advanced glycation end products,AGEs

Abbreviation

AGEs

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 μg/mL-10 μg/mL

Sensitivity

0.039 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	97
	Range %	92-103
1:4	Average %	99
	Range %	94-110
1:8	Average %	95
	Range %	87-100

Recovery

The recovery of mouse AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	84-99
EDTA plasma (n=4)	97	92-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














µg/ml	OD1	OD2	Average	Corrected
10	1.958	1.900	1.929	1.779
5	1.583	1.602	1.593	1.443
2.5	1.160	1.154	1.157	1.007
1.25	0.754	0.739	0.747	0.597
0.625	0.471	0.434	0.453	0.303
0.312	0.342	0.319	0.331	0.181
0.156	0.253	0.239	0.246	0.096
0	0.152	0.148	0.150

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse AGEs ELISA Kit was designed for the quantitative measurement of Mouse AGEs protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL .

γ-Glutamylcysteine suppresses epithelial-mesenchymal transition in lens epithelial cells to ameliorate diabetic cataract through regulating TGF-β2 and AMPK-related signaling pathways J Zhou, X Liu, L Zhao, C Yang, R Wang, L Sun,Journal of Functional Foods,2025
Guanxin Xiaoban capsules could treat atherosclerosis by affecting the gut microbiome and inhibiting the AGE–RAGE signalling pathway Y Dongliang,Journal of medical microbiology,2022
In vitro chronic glycation induces AGEs accumulation reducing insulin stimulated glucose uptake and increasing GLP1R in adipocytes NC Chilelli,American Journal of Physiology-Endocrinology and Metabolism,2021
Apigenin exhibits protective effects in a mouse model of D-galactose-induced aging via activating the Nrf2 pathway Ying Sang.et al,Food Funct.,2017
Pioglitazone ameliorates glomerular NLRP3 inflammasome activation in apolipoprotein E knockout mice with diabetes mellitus Wang Y.et al,PLoS One,2017
Targeted inhibition of RAGE reduces amyloid-B influx across the blood-brain barrier and improves cognitive deficits in db/db mice Wang H.et al,Neuropharmacology,2017
PPARr agonists regulate bidirectional transport of amyloid-B across the blood-brain barrier and hippocampus plasticity in db/db mice Wang H.et al,Br J Pharmacol.,2016
PPARr agonists regulate bidirectional transport of amyloid-B across the blood-brain barrier and hippocampus plasticity in db/db mice. Wang H.et al,Br J Pharmacol.,2016
Polydatin attenuates AGEs-induced upregulation of fibronectin and ICAM-1 in rat glomerular mesangial cells and db/db diabetic mice kidneys by inhibiting the activation of the SphK1-S1P signaling pathway Chen C.et al,Mol Cell Endocrinol.,2016
Antidiabetic drugs restore abnormal transport of amyloid-ß across the blood-brain barrier and memory impairment in db/db mice Chen F. et al,Neuropharmacology,2015

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■ Q&A

Regarding your ELISA kit CSB-E09414m, could you please advise on the material you used for the standard in this kit? What AGE is this kit detecting?

Thanks for your inquiry.
This kit detects total AGEs. The standard is polymer.
There are some protective components in the standard, but sorry we can not disclose the specific components.
Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.