Mouse advanced glycation end products,AGEs ELISA Kit

Code CSB-E09414m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse AGEs ELISA Kit was designed for the quantitative measurement of Mouse AGEs protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL .

Target Name advanced glycation end products,AGEs
Abbreviation AGEs
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.039 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 82-91  
1:2 Average % 97  
Range % 92-103  
1:4 Average % 99  
Range % 94-110  
1:8 Average % 95  
Range % 87-100  
Recovery
The recovery of mouse AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 84-99  
EDTA plasma (n=4) 97 92-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
             
             
             
             
             
             
             
             
             
             
             
             
             
µg/ml OD1 OD2 Average Corrected  
10 1.958 1.900 1.929 1.779  
5 1.583 1.602 1.593 1.443  
2.5 1.160 1.154 1.157 1.007  
1.25 0.754 0.739 0.747 0.597  
0.625 0.471 0.434 0.453 0.303  
0.312 0.342 0.319 0.331 0.181  
0.156 0.253 0.239 0.246 0.096  
0 0.152 0.148 0.150    
             
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

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 Q&A
Q:

Regarding your ELISA kit CSB-E09414m, could you please advise on the material you used for the standard in this kit? What AGE is this kit detecting?

A:
Thanks for your inquiry.
This kit detects total AGEs. The standard is polymer.
There are some protective components in the standard, but sorry we can not disclose the specific components.
Pls let me know if you have any further questions. Thank you.
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