Mouse advanced glycation end products,AGEs ELISA Kit

Code CSB-E09414m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
advanced glycation end products,AGEs
Abbreviation
AGEs
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 μg/mL-10 μg/mL
Sensitivity
0.039 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 82-91  
1:2 Average % 97  
Range % 92-103  
1:4 Average % 99  
Range % 94-110  
1:8 Average % 95  
Range % 87-100  
Recovery
The recovery of mouse AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 84-99  
EDTA plasma (n=4) 97 92-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
             
             
             
             
             
             
             
             
             
             
             
             
             
µg/ml OD1 OD2 Average Corrected  
10 1.958 1.900 1.929 1.779  
5 1.583 1.602 1.593 1.443  
2.5 1.160 1.154 1.157 1.007  
1.25 0.754 0.739 0.747 0.597  
0.625 0.471 0.434 0.453 0.303  
0.312 0.342 0.319 0.331 0.181  
0.156 0.253 0.239 0.246 0.096  
0 0.152 0.148 0.150    
             
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse AGEs ELISA Kit was designed for the quantitative measurement of Mouse AGEs protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL .

Citations

Customer Reviews and Q&A

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 Q&A
Q:

Regarding your ELISA kit CSB-E09414m, could you please advise on the material you used for the standard in this kit? What AGE is this kit detecting?

A:
Thanks for your inquiry.
This kit detects total AGEs. The standard is polymer.
There are some protective components in the standard, but sorry we can not disclose the specific components.
Pls let me know if you have any further questions. Thank you.
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