Rat advanced glycation end products,AGEs ELISA Kit

Code CSB-E09413r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Rat AGEs ELISA Kit was designed for the quantitative measurement of Rat AGEs protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.9 μg/mL-250 μg/mL and the sensitivity is 0.97 μg/mL .

Target Name advanced glycation end products,AGEs
Abbreviation AGEs
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.9 μg/mL-250 μg/mL
Sensitivity 0.97 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 86  
Range % 84-95  
1:10 Average % 95  
Range % 91-100  
1:20 Average % 85  
Range % 81-89  
1:40 Average % 93  
Range % 89-96  
The recovery of rat AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-96  
EDTA plasma (n=4) 95 90-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average Corrected  
250 2.194 2.286 2.240 2.084  
125 1.764 1.789 1.777 1.621  
62.5 1.270 1.256 1.263 1.107  
31.2 0.921 0.975 0.948 0.792  
15.6 0.455 0.465 0.460 0.304  
7.8 0.297 0.308 0.303 0.147  
3.9 0.248 0.233 0.241 0.085  
0 0.161 0.150 0.156    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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