Rat advanced glycation end products,AGEs ELISA Kit

Code CSB-E09413r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
advanced glycation end products,AGEs
Abbreviation
AGEs
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.9 μg/mL-250 μg/mL
Sensitivity
0.97 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 86  
Range % 84-95  
1:10 Average % 95  
Range % 91-100  
1:20 Average % 85  
Range % 81-89  
1:40 Average % 93  
Range % 89-96  
Recovery
The recovery of rat AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-96  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average Corrected  
250 2.194 2.286 2.240 2.084  
125 1.764 1.789 1.777 1.621  
62.5 1.270 1.256 1.263 1.107  
31.2 0.921 0.975 0.948 0.792  
15.6 0.455 0.465 0.460 0.304  
7.8 0.297 0.308 0.303 0.147  
3.9 0.248 0.233 0.241 0.085  
0 0.161 0.150 0.156    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat AGEs ELISA Kit was designed for the quantitative measurement of Rat AGEs protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.9 μg/mL-250 μg/mL and the sensitivity is 0.97 μg/mL .

Citations

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 Q&A
Q:

I'd like to order the "rat advanced glycation end products (AGEs) ELISA Kit (#CSB-E09413r)" from your company, but I want to know additional information:
Q1. Is there any reason (or evidence) why we select the AGEs Elisa Kit depending on the host species?
- Since AGEs do not have specific epitopes, I think that host species are not the critical factor to determine the antibody specificity of AGEs.
Q2. If I used the rat AGEs ELISA kit to analyze the mice tissue sample, is it hard to assure the specificity of this kit? (or data quality)

A:

A1: AGEs is a general name for a series of substances, instead of a single one. They have the same antigenic determinant. The reason why developed ELISA kits of different species is that the characteristics, anti-matrix-interference, and applicability of raw materials applied for different kits are different.
This kit CSB-E09413r (Rat advanced glycation end products, AGEs ELISA Kit) applies mouse monoclonal antibody and goat polyclonal antibody.
While this kit CSB-E09414m (Mouse advanced glycation end products, AGEs ELISA Kit) applies rat monoclonal antibody and goat polyclonal antibody.
Thus we suggest the user use the ELISA kit of the corresponding species to do the detection.

A2: This kit has no cross-activity with human and mouse AGEs recombinant. We tested the mouse blood sample with this kit and obtained no values. We do not suggest cross-species detection.

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