Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

Code CSB-E10787m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation Aβ1-42
Target Name amyloid beta peptide 1-42,Aβ1-42
Alias Amyloid beta 42(ABeta 42)
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %88-99
1:2Average %95
Range %85-105
1:4Average %96
Range %90-105
1:8Average %93
Range %86-100
Recovery
The recovery of mouse Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)9385-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.490 2.451 2.471 2.337
5002.049 1.985 2.017 1.883
2501.357 1.385 1.371 1.237
1250.866 0.837 0.852 0.718
62.50.517 0.524 0.521 0.387
31.20.347 0.355 0.351 0.217
15.60.244 0.228 0.236 0.102
00.135 0.132 0.134  
References
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Q&A and Customer Reviews

Q&A
Q:

Hi I would like to ask the following for the item Mouse amyloid beta peptide 1-40 ELISA kits:
1. How may test samples can be performed in 1 kit, is it possible to use the kit for 1 96-well plate

A:
Thanks for your inquiry. This is a 96T sandwich ELISA kit (double-antibody). For a 96T sandwich ELISA kit we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.
Customer Reviews

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