Mouse angiotensinogen (aGT) ELISA Kit

Instructions
Code CSB-E08566m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Alternative Names Agt ELISA kit; Serpina8Angiotensinogen ELISA kit; Serpin A8) [Cleaved into: Angiotensin-1 ELISA kit; Angiotensin 1-10 ELISA kit; Angiotensin I ELISA kit; Ang I); Angiotensin-2 ELISA kit; Angiotensin 1-8 ELISA kit; Angiotensin II ELISA kit; Ang II); Angiotensin-3 ELISA kit; Angiotensin 2-8 ELISA kit; Angiotensin III ELISA kit; Ang III ELISA kit; Des-Asp[1]-angiotensin II); Angiotensin-4 ELISA kit; Angiotensin 3-8 ELISA kit; Angiotensin IV ELISA kit; Ang IV); Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4] ELISA kit
Abbreviation AGT
Uniprot No. P11859
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse aGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 97
Range % 92-101
1:200 Average % 102
Range % 98-110
1:400 Average % 91
Range % 86-95
1:800 Average % 93
Range % 89-97
Recovery
The recovery of mouse aGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-93
EDTA plasma (n=4) 84 80-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 2.584 2.445 2.515 2.410
100 1.692 1.573 1.633 1.528
50 0.951 1.016 0.984 0.879
25 0.647 0.662 0.655 0.550
12.5 0.359 0.339 0.349 0.244
6.25 0.236 0.221 0.229 0.124
3.12 0.183 0.187 0.185 0.080
0 0.102 0.108 0.105  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.; FUNCTION
Subcellular Location Secreted
Protein Families Serpin family
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

STRING: 10090.ENSMUSP00000066488

UniGene: Mm.301626

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