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Mouse angiotensinogen (aGT) ELISA Kit

Citations (7) Protocol
Code CSB-E08566m
Size 96T,5×96T,10×96T
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Product Details

Target Name
angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Alternative Names
Agt ELISA kit; Serpina8Angiotensinogen ELISA kit; Serpin A8) [Cleaved into: Angiotensin-1 ELISA kit; Angiotensin 1-10 ELISA kit; Angiotensin I ELISA kit; Ang I); Angiotensin-2 ELISA kit; Angiotensin 1-8 ELISA kit; Angiotensin II ELISA kit; Ang II); Angiotensin-3 ELISA kit; Angiotensin 2-8 ELISA kit; Angiotensin III ELISA kit; Ang III ELISA kit; Des-Asp[1]-angiotensin II); Angiotensin-4 ELISA kit; Angiotensin 3-8 ELISA kit; Angiotensin IV ELISA kit; Ang IV); Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4] ELISA kit
Abbreviation
AGT
Uniprot No.
P11859
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse aGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:100 Average % 97
Range % 92-101
1:200 Average % 102
Range % 98-110
1:400 Average % 91
Range % 86-95
1:800 Average % 93
Range % 89-97
Recovery
The recovery of mouse aGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-93
EDTA plasma (n=4) 84 80-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 2.584 2.445 2.515 2.410
100 1.692 1.573 1.633 1.528
50 0.951 1.016 0.984 0.879
25 0.647 0.662 0.655 0.550
12.5 0.359 0.339 0.349 0.244
6.25 0.236 0.221 0.229 0.124
3.12 0.183 0.187 0.185 0.080
0 0.102 0.108 0.105 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse AGT ELISA Kit was designed for the quantitative measurement of Mouse AGT protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

Citations

Related Products

Agt Proteins

See all products about Agt >>

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Target Background

Function
(From Uniprot)
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.; acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.; stimulates aldosterone release.; is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
Subcellular Location
Secreted.
Protein Families
Serpin family
Tissue Specificity
Expressed by the liver and secreted in plasma.
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Related pathways
Renin-angiotensin system
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