Home
Kit
ELISA Kit
Mouse coagulation factor Ⅶ,FⅦ ELISA Kit

Mouse coagulation factor Ⅶ,FⅦ ELISA Kit

Protocol MSDS

Code	CSB-E17817m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Target Name

coagulation factor VII (serum prothrombin conversion accelerator)

Alternative Names

F7 ELISA Kit; Cf7 ELISA Kit; Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit

Abbreviation

Uniprot No.

P70375

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.45 ng/mL-30 ng/mL

Sensitivity

0.11 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Blood Coagulation

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅦ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	92
	Range %	86-96
1:2000	Average %	97
	Range %	92-102
1:4000	Average %	93
	Range %	88-97
1:8000	Average %	89
	Range %	84-94

Recovery

The recovery of mouse FⅦ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-95
EDTA plasma (n=4)	108	103-114

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.468	2.445	2.457	2.324
15	2.115	1.951	2.033	1.900
7.5	1.503	1.440	1.472	1.339
3.75	0.845	0.790	0.818	0.685
1.8	0.626	0.593	0.610	0.477
0.9	0.381	0.362	0.372	0.239
0.45	0.274	0.252	0.263	0.130
0	0.137	0.129	0.133

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse Coagulation factor VII (F7) ELISA Kit was designed for the quantitative measurement of Mouse Coagulation factor VII (F7) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.

Gene References into Functions

Leu8 is crucial for FVIIa-EPCR binding; this study characterizes its interaction in vivo in mice PMID: 28035745
FVIIa-antithrombin but not FVIIa is a ligand for LRP1, and LRP1 contributes to the clearance of FVIIa-antithrombin in vivo PMID: 27614059
FVIIa binding to EPCR leads to a barrier protective effect in vivo PMID: 24977291
Murine FVIIa binds poorly to murine EPCR. PMID: 22370814
GlycoPEGylated rFVIIa (N7-GP) has a prolonged hemostatic effect in hemophilic mice compared with rFVIIa PMID: 21366858
Conclude that the fVII-targeted verteporfin photodynamic therapy that we report here is a novel and effective therapeutic with improved selectivity for the treatment of breast cancer. PMID: 20504328
Recombinant FVIIa readily associates with the vascular endothelium and subsequently enters into extravascular spaces where it is likely to bind to tissue factor and is retained for extended time periods. PMID: 19943873
true circadian rhythms for FVII were found PMID: 15604416
Data suggest that long-term expression of murine activated factor VII is safe, but elevated levels cause premature mortality. PMID: 18398505
FVIIa functions in the asthmatic response to an allergen by stimulating lung eosinophilia, airway hyperresponsiveness, and mucin production. PMID: 19286924
Plasma elimination kinetics for factor VII are independent of its activation to factor VIIa and complex formation with plasma inhibitors. PMID: 19404533

Hide All

Subcellular Location

Secreted.

Protein Families

Peptidase S1 family

Tissue Specificity

Plasma and liver.

Database Links

KEGG: mmu:14068

STRING: 10090.ENSMUSP00000033820

UniGene: Mm.4827

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

The Overview of One Category of Important Cell Receptors: Receptor Tyrosine Kinases Cellular Senescence and Its Markers TPBG/5T4: the Oncofetal Protein, an Appealing Target for ADC or Vaccine Therapeutics in Tumors! ERBB3/HER3: a New Target for Tumor Therapy, a Driver Gene of Multi-Tumors CLDN4: A Potential Prognostic Indicator and Therapeutic Target in Tumors！ Oct4 -- The Gatekeeper of Pluripotency of Embryonic Stem Cells What Do You Need to Know Before Researching Human Metapneumovirus? A Comprehensive Exploration into Amyotrophic Lateral Sclerosis ACVRL1 (Alk1): a Member of the TGF-β Receptor Family, a Novel Target for Anti-Angiogenic Drug Research! Tumor Necrosis Factor Receptor Superfamily and Apoptosis The Overview of Coagulation Monoclonal Antibody Drugs Overview Exploring Neurodegeneration: A Comprehensive Guide for Researchers GIPR: the Crucial Regulator of Glucose and Lipid Metabolism in Diabetes and Obesity! CD70 (CD27L/TNFSF7): a TNF Receptor Member, a “Star” Target for Clinical Research in Systemic Lupus Erythematosus (SLE), Arthritis, and Tumors! Diabetes: What You Need to Know Before Starting Research? Does TNF Superfamily Inhibit Cancer or Promote Its Development? GLP-1R: An Energy Metabolism Regulator of GPCR Family, an Effective Drug Target for Type 2 Diabetes Therapy! The Overview of Colony Stimulating Factors The Overview of Growth Factor Tumor Necrosis Factor (TNF) A Group of Promising Proteins - Cell Cycle Markers The Overview of Interleukin Do You Really Know TGF-β Superfamily? ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Related pathways

Complement and coagulation cascades

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Mouse coagulation factor Ⅶ,FⅦ ELISA Kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background