Mouse granzyme A (GZMA) ELISA Kit

Code CSB-E08717m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse GZMA ELISA Kit was designed for the quantitative measurement of Mouse GZMA protein in serum, plasma,tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Target Name granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
Alternative Names Gzma ELISA Kit; Ctla-3 ELISA Kit; Ctla3 ELISA Kit; Mtsp-1 ELISA Kit; Granzyme A ELISA Kit; EC 3.4.21.78 ELISA Kit; Autocrine thymic lymphoma granzyme-like serine protease ELISA Kit; CTLA-3 ELISA Kit; Fragmentin-1 ELISA Kit; T cell-specific serine protease 1 ELISA Kit; TSP-1 ELISA Kit
Abbreviation GZMA
Uniprot No. P11032
Species Mus musculus (Mouse)
Sample Types serum, plasma,tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.
Gene References into Functions
  1. GzmA and gzmB were predominantly expressed by natural killer cells, and during abdominal sepsis, the percentage of these cells expressing gzms in peritoneal lavage fluid decreased, while the amount of expression in the gzm(+) cells increased PMID: 28694562
  2. a novel endogenous trigger for autoimmune diabetes and an in vivo role for granzyme A in maintaining immune tolerance. PMID: 28733313
  3. this study shows that gzmA and gzmB partly regulate local inflammation during early pneumonia but eventually play an insignificant role during pneumosepsis by the common human pathogen Klebsiella pneumoniae PMID: 26894590
  4. Granzyme A does not have a crucial role in vivo in the protective response to tuberculosis. PMID: 27055232
  5. Data indicate N-terminomics on the human and mouse granzymes A and K by combined fractional diagonal chromatography (COFRADIC). PMID: 25383893
  6. The results, in susceptible B6 mice for GzmB and in resistant 129/Sv mice for GzmA and/or the GzmB cluster, point to granzyme-mediated host defense regulation in the liver in experimental visceral leishmaniasis. PMID: 25452549
  7. Estrogen increases the extracellular expression and interleukin (IL)-12-induced activity of a critical member of serine protease family granzyme A. PMID: 24840346
  8. functional divergence between human and mouse granzyme A PMID: 24505135
  9. We have demonstrated that cell death can be induced when mGzmA is delivered by primary natural killer cells via authentic Cytotoxic lymphocyte/target cell interactions. PMID: 23744295
  10. Regulated secretion of granzyme A and cytotoxic killing was enhanced and correlated with increased vesicle-associated membrane protein 7 availability. PMID: 23084031
  11. GzmA deficiency in filarial infection is linked with reduced inflammation and a trend toward increased alternatively activated macrophages. PMID: 21248253
  12. granzyme A- and B-cluster deficiency delays the acute progression of pneumovirus disease by reducing alveolar injury. PMID: 20018616
  13. the genes for perforin, the three major T cell granzymes (A-C) and IFN-gamma are differentially expressed during primary activation of naive CD8(+) T cells, kinetically and at the single-cell level PMID: 12039912
  14. granzyme A and granzyme B have a similar potential to induce rapid perf-mediated apoptosis but that their individual contribution to the underlying intracellular process is dictated by the quality of the target cell PMID: 12115618
  15. NK cell mediated tumor control is dependent on granzymes. PMID: 12355441
  16. some cytolytic T lymphocytes mature into perforin/granzyme-expressing effector cells in the mediastinal lymph node and are detectable when they accumulate in lung infection PMID: 12601154
  17. In all tumor models examined thus far, granzyme A is not necessary for tumor rejection mediated by the perforin pathway in vivo. PMID: 12847210
  18. gzmA and gzmB induce multiple independent cell death pathways; all gzm-induced apoptotic features analyzed depend critically on perf. PMID: 15534000
  19. polymorphonuclear leukocytes from mice and humans lack the 3 cytotoxic effector molecules, gzmA, granzyme B, and perforin, generally associated with natural killer and cytotoxic T lymphocytes PMID: 15998831
  20. IL-2 increased the expression of perforin and granzyme A, B, and C mRNA; induction of granzyme A, B, and C mRNA required exogenous IL-2, whereas induction of perforin and IFN-gamma expression did not. PMID: 16339537
  21. CTLs from granzyme AB(-/-) mice induce target cell death by a unique mechanism that is distinct from both perforin lysis and apoptosis. PMID: 16606695
  22. Mouse granzyme B is 30 times less cytotoxic than human granzyme B and does not require Bid for killing but regains cytotoxicity on engineering of its active site cleft. Mouse granzyme A is considerably more cytotoxic than human granzyme A. PMID: 17116752
  23. Skin-, but not lung-associated primary mast cells as well as in vitro-differentiated bone marrow-derived mast cells (BMMC) express granzyme (gzm) B, but not gzmA or perforin (perf). PMID: 17599099
  24. GzmA accesses the mitochondrial matrix to cleave the complex I protein NDUFS3, an iron-sulfur subunit of the NADH:ubiquinone oxidoreductase complex I. PMID: 18485875
  25. grzK plays an important role in CD8(+) T-cell cytotoxicity both in the presence and absence of grzA and B. PMID: 18788942
  26. the granule secretory pathway plays an unexpected role in inflammation, with GzmA acting as an endogenous modulator. PMID: 18951048
  27. Neither gzmA nor gzmB is required for rapid and efficient in vivo cytotoxicity by cytotoxic T (Tc) cells. PMID: 19525394

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Subcellular Location Secreted. Cytoplasmic granule.
Protein Families Peptidase S1 family, Granzyme subfamily
Tissue Specificity Found in cytotoxic lymphocytes and in normal lymphoid tissues such as thymus and spleen.; [Isoform HF1]: More abundant in lymphoid tissues than isoform HF2.
Database Links

KEGG: mmu:14938

STRING: 10090.ENSMUSP00000023897

UniGene: Mm.15510

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