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Mouse intestinal fatty acid binding protein,iFABP ELISA Kit

Instructions
Code CSB-E08025m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name intestinal fatty acid binding protein,iFABP
Alternative Names Fabp2 ELISA Kit; FabpiFatty acid-binding protein ELISA Kit; intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; I-FABP ELISA Kit
Abbreviation iFABP
Uniprot No. P55050
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:100Average %102
Range %96-108
1:200Average %95
Range %91-99
1:400Average %96
Range %91-102
1:800Average %86
Range %82-92
Recovery
The recovery of mouse iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8882-94
EDTA plasma (n=4)9690-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.217 2.317 2.267 2.163
2001.367 1.467 1.417 1.313
1000.777 0.797 0.787 0.683
500.412 0.422 0.417 0.313
250.265 0.268 0.267 0.163
12.50.189 0.199 0.194 0.090
6.250.162 0.165 0.164 0.060
00.104 0.103 0.104 　
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Gene References into Functions
  1. This direct comparison provides evidence that LFABP and IFABP have distinct roles in intestinal lipid metabolism; differential intracellular functions in intestine and in liver PMID: 23990461
  2. detected the common as well as sex-differential pathways that are modified due to the loss of Fabp2. These findings suggest that the pathways involved in nutrient and xenobiotic metabolism in the intestine are regulated by sex-specific mechanisms PMID: 22584290
  3. loss of I-FABP renders male mice sensitive to high fat diet-induced fatty liver, and this effect is independent of hepatic L-FABP PMID: 17905650
Subcellular Location Cytoplasm
Protein Families Calycin superfamily, Fatty-acid binding protein (FABP) family
Tissue Specificity Expressed in the small intestine. Highest expression levels in the proximal ileum.
Database Links

KEGG: mmu:14079

STRING: 10090.ENSMUSP00000023820

UniGene: Mm.28398
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