Mouse intestinal fatty acid binding protein,iFABP ELISA Kit

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Mouse intestinal fatty acid binding protein,iFABP ELISA Kit

Name: Mouse intestinal fatty acid binding protein,iFABP ELISA Kit
Brand: CUSABIO
SKU: CSB-E08025m

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Code	CSB-E08025m
Size	96T,5×96T,10×96T
Trial Size
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Target Background

Target Name

intestinal fatty acid binding protein,iFABP

Alternative Names

Fabp2 ELISA Kit; FabpiFatty acid-binding protein ELISA Kit; intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; I-FABP ELISA Kit

Abbreviation

iFABP

Uniprot No.

P55050

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	102
	Range %	96-108
1:200	Average %	95
	Range %	91-99
1:400	Average %	96
	Range %	91-102
1:800	Average %	86
	Range %	82-92

Recovery

The recovery of mouse iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-94
EDTA plasma (n=4)	96	90-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.217	2.317	2.267	2.163
200	1.367	1.467	1.417	1.313
100	0.777	0.797	0.787	0.683
50	0.412	0.422	0.417	0.313
25	0.265	0.268	0.267	0.163
12.5	0.189	0.199	0.194	0.090
6.25	0.162	0.165	0.164	0.060
0	0.104	0.103	0.104

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Novel role of hesperidin improve obesity in HFD mice by modulating the composition of the gut microbiota Z Liu,Research Square Preprint,2020

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Function (From Uniprot)	FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Gene References into Functions	This direct comparison provides evidence that LFABP and IFABP have distinct roles in intestinal lipid metabolism; differential intracellular functions in intestine and in liver PMID: 23990461 detected the common as well as sex-differential pathways that are modified due to the loss of Fabp2. These findings suggest that the pathways involved in nutrient and xenobiotic metabolism in the intestine are regulated by sex-specific mechanisms PMID: 22584290 loss of I-FABP renders male mice sensitive to high fat diet-induced fatty liver, and this effect is independent of hepatic L-FABP PMID: 17905650
Subcellular Location	Cytoplasm
Protein Families	Calycin superfamily, Fatty-acid binding protein (FABP) family
Tissue Specificity	Expressed in the small intestine. Highest expression levels in the proximal ileum.
Database Links	KEGG: mmu:14079 STRING: 10090.ENSMUSP00000023820 UniGene: Mm.28398

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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