Mouse oxidized low density lipoprotein,OxLDL ELISA Kit

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Name: Mouse oxidized low density lipoprotein,OxLDL ELISA Kit
Brand: CUSABIO
SKU: CSB-E07933m

^Instructions

Code	CSB-E07933m
Size	96T,5×96T,10×96T
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Target Name

oxidized lowdensity lipoprotein,OxLDL

Abbreviation

OxLDL

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.45 nmol/mL-30 nmol/mL

Sensitivity

0.11 nmol/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-95
1:200	Average %	97
	Range %	92-103
1:400	Average %	85
	Range %	81-87
1:800	Average %	89
	Range %	83-96

Recovery

The recovery of mouse OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	95	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nmol/ml	OD1	OD2	Average	Corrected
30	2.648	2.772	2.710	2.571
15	2.139	2.130	2.135	1.996
7.5	1.527	1.639	1.583	1.444
3.75	0.885	0.866	0.876	0.737
1.86	0.541	0.532	0.537	0.398
0.94	0.378	0.405	0.392	0.253
0.45	0.243	0.242	0.243	0.104
0	0.136	0.142	0.139

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Defined p16High Senescent Cell Types Are Indispensable for Mouse Healthspan L Grosse,Cell Metabolism,2020
Exogenous GM3 ganglioside inhibits atherosclerosis via multiple steps: A potential atheroprotective drug Ao M, et al,Pharmacological Research,2019
Sex-dependent co-occurrence of hypoxia and β-amyloid plaques in hippocampus and entorhinal cortex is reversed by long-term treatment with ubiquinol and ascorbic acid in the 3 × Tg-AD mouse model of Alzheimer's disease Javier.et al,Molecular and Cellular Neuroscience,2018
ox-LDL induces endothelial dysfunction by promoting Arp2/3 complex expression Tang Y.et al,Biochem Biophys Res Commun.,2016
Proatherosclerotic Effect of the a1-Subunit of Soluble Guanylyl Cyclase by Promoting Smooth Muscle Phenotypic Switching. Segura-Puimedon M.et al,Am J Pathol.,2016
Low ambient oxygen prevents atherosclerosis. Kang JG. et al,J Mol Med (Berl).,2016
Orange beverage ameliorates high-fat-diet-induced metabolic disorder in mice Escudero-Lpez B.et al,Journal of Functional Foods,2016
Inhibition of Glutathione Production Induces Macrophage CD36 Expression and Enhances Cellular-oxidized Low Density Lipoprotein (oxLDL) Uptake. Yang X.et al,J Biol Chem.,2015
P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation Peng K et al,Int J Mol Med,2015
Effects of flavonol-rich green tea cultivar (Camellia sinensis L.) on plasma oxidized LDL levels in hypercholesterolemic mice Nomura S. et al,Biosci Biotechnol Biochem,2015
Sublingual Vaccine with GroEL Attenuates Atherosclerosis Hagiwara M et al,J Dent Res,2014
Oxidatively modified LDL particles in the human placenta in early and late onset intrauterine growth restriction Ulrich Pecks et al,Placenta,2013
Periodontal Pathogen Accelerates Lipid Peroxidation and Atherosclerosis Jia R et al,J Dent Res,2013
Lactobacillus acidophilus ATCC 4356 attenuates the atherosclerotic progression through modulation of oxidative stress and inflammatory process Chen L et al,Int Immunopharmacol,2013
Increased Blood-Brain Barrier Permeability and Brain Edema After Focal Cerebral Ischemia Induced by Hyperlipidemia Ayman ElAli et al,Stroke,2011

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

■ Q&A

I would like to know which portion of OXLDL is recognized by your mouse oxLDL kit cat# CSB-E07933m.

Thanks for your inquiry.
The standard of this kit is OXLDL prepared by the oxidation of cupric sulfate.
IIt can detect endogenous OXLDL.
At the same time, the OXLDL prepared by different methods can be detected as long as the corresponding recognition sites/regions are available.
Sorry we are not clear about the specific recognition area. If you want to detect other methods to prepare the OXLDL, we suggest you do a preliminary experiment.

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