Mouse oxidized low density lipoprotein,OxLDL ELISA Kit

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Name: Mouse oxidized low density lipoprotein,OxLDL ELISA Kit
Brand: CUSABIO
SKU: CSB-E07933m

Product Details

Description

This Mouse OxLDL ELISA Kit was designed for the quantitative measurement of Mouse OxLDL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 nmol/mL-30 nmol/mL and the sensitivity is 0.11 nmol/mL .

Target Name

oxidized lowdensity lipoprotein,OxLDL

Abbreviation

OxLDL

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.45 nmol/mL-30 nmol/mL

Sensitivity

0.11 nmol/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-95
1:200	Average %	97
	Range %	92-103
1:400	Average %	85
	Range %	81-87
1:800	Average %	89
	Range %	83-96

Recovery

The recovery of mouse OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	95	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nmol/ml	OD1	OD2	Average	Corrected
30	2.648	2.772	2.710	2.571
15	2.139	2.130	2.135	1.996
7.5	1.527	1.639	1.583	1.444
3.75	0.885	0.866	0.876	0.737
1.86	0.541	0.532	0.537	0.398
0.94	0.378	0.405	0.392	0.253
0.45	0.243	0.242	0.243	0.104
0	0.136	0.142	0.139

Materials provided

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse OxLDL antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled OxLDL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Code	CSB-E07933m
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.