Pig oxidized low density lipoprotein,OxLDL ELISA Kit

Code CSB-E06527p
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation OxLDL
Target Name oxidized lowdensity lipoprotein,OxLDL
Species Sus scrofa (Pig)
Sample Types serum, plasma
Detection Range 0.39 nmol/mL-100 nmol/mL
Sensitivity 0.39 nmol/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:200 Average % 97    
Range % 92-105    
1:400 Average % 95    
Range % 83-100    
1:800 Average % 95    
Range % 89-100    
1:1600 Average % 89    
Range % 85-99    
Recovery
The recovery of pig OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 90-100    
EDTA plasma (n=4) 94 87-98    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
nmol/ml OD1 OD2 Average      
100 0.349 0.342 0.346      
25 0.627 0.638 0.633      
6.25 0.925 0.914 0.92      
1.56 1.136 1.153 1.145      
0.39 1.594 1.625 1.61      
0 1.899 1.864 1.882      
References
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