Human oxidized low density lipoprotein,OxLDL ELISA Kit

Code CSB-E07931h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human OxLDL ELISA Kit was designed for the quantitative measurement of Human OxLDL protein in serum, plasma, urine, saliva, tissue homogenates, cell lysates. It is a Competitive ELISA kit, its detection range is 1.56 mU/mL-100 mU/mL and the sensitivity is 0.78 mU/mL.

Target Name oxidized lowdensity lipoprotein,OxLDL
Abbreviation OxLDL
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, saliva, tissue homogenates, cell lysates
Detection Range 1.56 mU/mL-100 mU/mL
Sensitivity 0.78 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 99  
Range % 93-105  
1:200 Average % 94  
Range % 91-98  
1:400 Average % 103  
Range % 99-107  
1:800 Average % 86  
Range % 80-92  
Recovery
The recovery of human OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 94-104  
EDTA plasma (n=4) 94 88-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average    
100 0.130 0.129 0.130    
50 0.306 0.301 0.304    
25 0.549 0.557 0.553    
12.5 0.941 0.956 0.949    
6.25 1.401 1.418 1.410    
3.12 1.953 1.967 1.960    
1.56 2.186 2.087 2.137    
0 2.758 2.656 2.707    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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