Rat oxidized low density lipoprotein,OxLDL ELISA Kit

Rat oxidized low density lipoprotein,OxLDL ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Rat oxidized low density lipoprotein,OxLDL ELISA Kit

Citations (2) Protocol MSDS

Code	CSB-E07932r
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (2)
Related Products
Customer Reviews and Q&A

Target Name

oxidized lowdensity lipoprotein,OxLDL

Abbreviation

OxLDL

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-96
1:200	Average %	94
	Range %	90-99
1:400	Average %	100
	Range %	96-106
1:800	Average %	90
	Range %	85-96

Recovery

The recovery of rat OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-100
EDTA plasma (n=4)	91	89-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.668	2.783	2.726	2.541
200	2.296	2.284	2.290	2.105
100	1.704	1.735	1.720	1.535
50	1.076	1.083	1.080	0.895
25	0.607	0.624	0.616	0.431
12.5	0.408	0.417	0.413	0.228
6.25	0.291	0.298	0.295	0.110
0	0.184	0.186	0.185

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat OxLDL ELISA Kit was designed for the quantitative measurement of Rat OxLDL protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

TetraSOD®, a Unique Marine Microalgae Ingredient, Promotes an Antioxidant and Anti-Inflammatory Status in a Metabolic Syndrome-Induced Model in Rats K Gil-Cardoso,Nutrients,2022
Longevity, tumor, and physical vitality in rats consuming ginsenoside Rg1 CC Hsieh,Journal of Ginseng Research,2021

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.