Mouse plasma kallikrein(KLKB1) ELISA Kit

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Mouse plasma kallikrein(KLKB1) ELISA Kit

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Protocol

Code	CSB-E16637m-IS
Size	96T,5×96T,10×96T
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Product Details
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Customer Reviews and Q&A
Target Background

Target Name

plasma kallikrein(KLKB1)

Alternative Names

Klkb1 ELISA Kit; Klk3 ELISA Kit; PkPlasma kallikrein ELISA Kit; EC 3.4.21.34 ELISA Kit; Fletcher factor ELISA Kit; Kininogenin ELISA Kit; Plasma prekallikrein) [Cleaved into: Plasma kallikrein heavy chain; Plasma kallikrein light chain] ELISA Kit

Abbreviation

KLKB1

Uniprot No.

P26262

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.3125 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:2000	Average %	92
	Range %	88-98
1:4000	Average %	88
	Range %	82-92
1:8000	Average %	97
	Range %	92-106
1:16000	Average %	98
	Range %	92-105

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
40	2.745	2.662	2.704	2.698
20	1.974	2.012	1.993	1.988
10	1.334	1.285	1.309	1.304
5	0.869	0.866	0.867	0.862
2.5	0.594	0.584	0.589	0.584
1.25	0.343	0.363	0.353	0.348
0.625	0.191	0.187	0.189	0.184
0	0.006	0.006	0.006

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Gene References into Functions

Plasma kallikrein mediates brain hemorrhage and edema caused by tissue plasminogen activator therapy in mice after stroke. PMID: 28130211
we found that post-ejaculated semen from fertile wild-type males was solidified and the sperm were entrapped in Wnt7aCre/+;Esr1f/f uteri, compared to the watery semen (liquefied) found in Esr1f/f controls.Kallikrein-related peptidases 3 (KLK3) and other kallikrein-related peptidases from male prostate glands are responsible for semen liquefaction by cleaving gel-forming proteins PMID: 28414719
Plasma kallikrein deletion prevents occlusive thrombus formation in mice with a minimal role in provoked bleeding. PMID: 22398951
PKK or fXII deficiency reduced thrombus formation in both arterial and venous thrombosis models, without an apparent effect on hemostasis. PMID: 21821705
the prekallikrein gene (Klkb1) is expressed highly in the mammary gland during stromal remodeling periods including puberty and postlactational involution. PMID: 19297327

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Subcellular Location

Secreted.

Protein Families

Peptidase S1 family, Plasma kallikrein subfamily

Database Links

KEGG: mmu:16621

STRING: 10090.ENSMUSP00000112174

UniGene: Mm.482691

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Email: [email protected]
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Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Related pathways

Complement and coagulation cascades

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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