Mouse soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL ELISA KIT

Instructions
Code CSB-E07371m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name tumor necrosis factor (ligand) superfamily, member 10
Alternative Names Tnfsf10 ELISA Kit; Trail ELISA Kit; Tumor necrosis factor ligand superfamily member 10 ELISA Kit; TNF-related apoptosis-inducing ligand ELISA Kit; Protein TRAIL ELISA Kit; CD antigen CD253 ELISA Kit
Abbreviation TNFSF10
Uniprot No. P50592
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse sTRAIL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 81-91  
1:2 Average % 98  
Range % 93-104  
1:4 Average % 92  
Range % 90-95  
1:8 Average % 94  
Range % 86-97  
Recovery
The recovery of mouse sTRAIL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-97  
EDTA plasma (n=4) 96 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.887 2.812 2.850 2.677  
100 2.382 2.311 2.347 2.174  
50 1.669 1.639 1.654 1.481  
25 0.911 0.878 0.895 0.722  
12.5 0.595 0.561 0.578 0.405  
6.25 0.401 0.388 0.395 0.222  
3.12 0.312 0.307 0.310 0.137  
0 0.175 0.171 0.173    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Gene References into Functions
  1. TRAIL protects against insulin resistance, NAFLD and vascular inflammation. Increasing TRAIL levels may be an attractive therapeutic strategy, to reduce features of diabetes, as well as liver and vascular injury, so commonly observed in individuals with NAFLD. PMID: 28507343
  2. Isolated highly-pure population of DcR2-positive renal tubular epithelial cells was isolated by MACS, which was confirmed to comprise of active senescent RTECs based on the cell cycle phase. PMID: 28461078
  3. Data suggest that the CXCL9-CXCR3 axis plays a pivotal role in the liver-specific distribution of TRAIL+ NK cells in mice. PMID: 29088306
  4. this study shows pathogenic role for TRAIL in chronic obstructive pulmonary disease PMID: 26555706
  5. pCLE results indicated that endomicroscopy could effectively quantify injected MSCs that homed to subcutaneous xenograft tumor sites in vivo and correlated well with the therapeutic effects of the TRAIL gene PMID: 27617958
  6. the aim of this study is to elucidate the role of TRAIL during rhinovirus (RV) infection in vivo. PMID: 27836899
  7. Tnfsf10 expression is increased in eosinophilic esophagitis. Tnfsf10(-/-) mice were largely protected from esophageal fibrosis and eosinophilic inflammation. PMID: 27742702
  8. Diabetes significantly increased OPG and the OPG/TRAIL ratio expression in the aorta, while dyslipidemia was the major determinant of the changes observed in the heart, where it significantly increased OPG and reduced TRAIL expression, thus increasing cardiac OPG/TRAIL ratio. PMID: 28070143
  9. Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology.( PMID: 26935795
  10. TRAIL can promote angiogenesis following hindlimb ischemia in vivo via NOX4/eNOS/nitric oxide signaling. PMID: 26572549
  11. PARP1 acts as a prominent upstream regulator of high mobility group box 1-mediated autophagy and maintains a homeostatic balance between apoptosis and autophagy, which provides new insight into the mechanism of TNFSF10 resistance PMID: 25607248
  12. Data suggest that Socs3 (suppressor of cytokine signaling 3) plays critical negative role in regulation of Trail expression in endoplasmic reticulum stress in macrophages via Jun N-terminal kinase/AP-1 transcription factor signaling. PMID: 25827060
  13. These findings indicate that acute fasting enhances TRAIL-mediated liver natural killer cell activity against neoplastic cells through upregulation of heat shock protein 70. PMID: 25356750
  14. These results demonstrated that TRAIL plays a deleterious role in acute kidney injury pathogenesis induced by scald burns PMID: 25031778
  15. TRAIL-deficiency in ApoE(-/-) mice exacerbates nephropathy and insulin resistance PMID: 24667560
  16. TRAIL induces apoptosis in tissue-infiltrating neutrophils thereby protecting organs from sepsis-induced injury. PMID: 24887152
  17. Tumor necrosis factor-related apoptosis-inducing ligand translates neonatal respiratory infection into chronic lung disease. PMID: 24045576
  18. These results suggest that TRAIL inhibits VEGF-induced angiogenesis by increasing caspase-8 activity and subsequently decreasing non-apoptotic signaling functions of procaspase-8, without inducing caspase-3 activation and endothelial cell cytotoxicity. PMID: 24097299
  19. expression of TRAIL on natural killer cells is protective in a murine model of hepatic ischemia-reperfusion injury through modulation of natural killer cell cytotoxicity and NK cell differentiation PMID: 24804996
  20. TRAIL signaling and cell death in the immature central nervous system after hypoxia-ischemia and inflammation PMID: 24509861
  21. The extracellular domain of Fn14 fused to the extracellular domain of the TRAIL ligand is designed to modulate the immune system as an anti-inflammatory agent. PMID: 23497038
  22. oncogene metadherin modulates the apoptotic pathway based on the tumor necrosis factor superfamily member TRAIL (Tumor Necrosis Factor-related Apoptosis-inducing Ligand) in breast cancer PMID: 23408429
  23. Data indicate that TNF-related apoptosis-inducing ligand knockout mice (TRAIL -/-) showed significantly decreased lung bacterial clearance and survival in response to Streptococcus pneumoniae. PMID: 23071253
  24. Data indicate the importance of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in pulmonary arterial hypertension (PAH) pathogenesis and suggest its potential as a therapeutic target to direct future translational therapies PMID: 23071256
  25. data demonstrate that TRAIL induces osteoclastic differentiation via a TRAF-6 dependent signaling pathway PMID: 22719861
  26. CD8+ T cells use TRAIL to restrict West Nile virus pathogenesis by controlling infection in neurons. PMID: 22740407
  27. findings shed light on the possible anti-adipogenic and anti-inflammatory effects of TRAIL and open new therapeutic possibilities against obesity, systemic inflammation and T2DM PMID: 22616837
  28. Epirubicin enhances the antiarthritic effect of rAAV2/5-TRAIL and that combination treatment might be an important therapeutic alternative, with practical significance for rheumatoid arthritis. PMID: 22131069
  29. It was shown for the first time that TRAIL deficiency promotes numerous features of diabetes typical of human disease, and are associated with reduced insulin and pancreatic inflammation/apoptosis. PMID: 21965021
  30. TRAIL strongly synergizes with APAP in inducing cell death in hepatocyte-like cells lines and primary hepatocyte. PMID: 21654829
  31. the tumoricidal activity of CnB-activated peritoneal macrophages is partially dependent on TRAIL. PMID: 22116828
  32. TRAIL regulates the magnitude of the influenza A virus (IAV)-specific CD8 T cell response during a clinically significant IAV infection to decrease the chance for infection-induced immunopathology. PMID: 21940678
  33. TRAIL-deficient mice had an enhanced inflammatory response with increased neutrophil numbers and reduced neutrophil apoptosis PMID: 21562052
  34. Data suggest that deficiency of TRADD sensitizes cells to TRAIL-induced apoptosis, and that enhanced cell death in TRADD(-/-) MEFs is associated with defective NF-kappaB activation. PMID: 21627969
  35. study concludes that TRAIL attenuates plaque size at early stages of atherosclerosis PMID: 21324463
  36. TRAIL and osteoprotegerin are expressed in atherosclerotic plaques in a calcification-prone mouse model PMID: 21426505
  37. Data show that the number of tumors observed in TRAIL transgenic animals was significantly lower than in the control animals, and the lesions observed were mostly benign. PMID: 21463519
  38. is not required for the development of peripheral lymphopenia or granuloma necrosis during infection with Mycobacterium avium PMID: 21470210
  39. resveratrol can enhance the apoptosis-inducing potential of TRAIL by activating FKHRL1 and its target genes PMID: 21209944
  40. CD8-positive regulatory T cells generated after anterior chamber injection of antigen via the retro-orbital plexus enforce systemic tolerance in a TRAIL-dependent manner to inhibit inflammation in the eye. PMID: 21169546
  41. TRAIL suppresses autoimmunity by two mechanisms: the inhibition of Th1 cells and the promotion of Tregs PMID: 20921531
  42. extrinsic and intrinsic pathways, via Trail/Dr5 and Puma respectively, could be engaged in distinct subpopulations of spermatogonia PMID: 20711434
  43. sTRAIL is inversely associated with the mortality risk in chronic kidney disease patients. PMID: 20190248
  44. Data show that in Abelson cells, the inhibition of both NF-kappaB and FoxO3a activity is required for suppression of TRAIL transcription and maintenance of the transformed state. PMID: 20213318
  45. TRAIL- and GITRL-activated pathways synergise in the development of SCI-related inflammatory damage. PMID: 20107429
  46. apoptotic cells generated during sepsis induced a CD8+ regulatory T cell that suppressed delayed-type hypersensitivity by TRAIL production PMID: 20483771
  47. results indicate that cells that survive TRAIL treatment may do so by activation of a TAK1-NF-kappaB pathway that drives expression of cFlipL, and suggest that TAK1 may be a good target for overcoming TRAIL resistance PMID: 20062539
  48. mice defective in both tumor necrosis factor-related apoptosis-inducing ligand and Fas ligand reveal new roles for TRAIL in lymphocyte homeostasis and autoimmune lymphoproliferative syndromes PMID: 20185587
  49. TRAIL induction involves FGF-2, Sp1-phosphorylation and NFkappaB and that TRAIL promotes VSMC proliferation and neointima formation after arterial injury. PMID: 20150555
  50. Data show that alpha-GalCer/AdmIL-2 treatment expanded the absolute numbers of lung and liver NK, NKT and T-cells as well as the TNF-related apoptosis-inducing ligand (TRAIL) expression of these cells. PMID: 19901518
  51. Data show that G-CSF treatment increased the amount of Fas-L, TRAIL and Bax proteins, whereas decreased the expression of procaspase 3 and Bcl-2. PMID: 19633419
  52. TRAIL plays a role in the control of retinal neovascularization PMID: 19893042
  53. The cytolytic molecules Fas ligand and TRAIL are required for murine thymic graft-versus-host disease. PMID: 19955659
  54. The use of TRAIL gene-targeted mice, devoid of TRAIL expression and TRAIL-mediated cytotoxicity, demonstrates the key role of TRAIL in suppressing tumor initiation and metastasis. PMID: 11801676
  55. TRAIL plays a critical role in the NK cell-mediated and IFN-gamma-dependent tumor surveillance PMID: 11805143
  56. TRAIL acts as the effector molecule that eliminates subcutaneously developing TRAIL-sensitive tumors. PMID: 12088418
  57. role of TRAIL in mouse mastocytoma PMID: 12209599
  58. TRAIL/Apo2L is important in controlling tumors in vivo, but is not an essential regulator of osteoprotegerin-mediated biology, under normal physiological conditions. PMID: 12209637
  59. Ocular tissue displays functional TRAIL as determined by in vitro killing of TRAIL-sensitive tumor cell lines. PMID: 12391182
  60. Mediates apoptosis is an important endogenous mechanism for resistance to liver metastases in murine renal cancer. PMID: 12517799
  61. GDVII virus induces apoptosis in murine astrocytes through tumor necrosis factor (TNF)-receptor and TNF-related apoptosis-inducing ligand PMID: 12842625
  62. TRAIL is an important immune regulator of the development of type 1 diabetes. PMID: 12882912
  63. Results suggest that TRAIL is not a critical factor for thymocyte negative selection. PMID: 12900523
  64. TRAIL deficiency accelerates autoimmune diabetes and enhances autoimmune responses. PMID: 12941766
  65. sensitization to TRAIL requires p53 PMID: 14645705
  66. Immature dendritic cells (DC) are rapidly eliminated by natural killer cells in vivo via a pathway dependent on TRAIL, highlighting the potential importance of innate effector mechanisms that limit DC-based vaccination. PMID: 14688317
  67. plays a crucial role in hepatic cell death and hepatic inflammation PMID: 14702109
  68. TRAIL appears to play an important role in corneal-allograft rejection and may be used to prolong the survival of allografts. PMID: 14702523
  69. matrix protein of lyssaviruses plays a major role in the early induction of TRAIL-mediated apoptosis PMID: 15163747
  70. TRAIL blocks osteoclastic differentiation induced by RANKL plus M-CSF PMID: 15213096
  71. Trail plays a crucial role in Listeria-induced apoptosis and listeriosis. PMID: 15494516
  72. we demonstrate that TRAIL is the dominant cytotoxic effector molecule expressed by NK cells in fetal mice; a phenotypically immature TRAIL+ NK cell subpopulation is retained in the liver of adult mice, and its retention is dependent on IFN-gamma PMID: 15536146
  73. Regulation of Trail expression can therefore account for the role of CD4+ T cells in the generation of CD8+ T cell memory and represents a novel mechanism for controlling adaptive immune responses PMID: 15744305
  74. plays an important role in the immune response to influenza virus infection PMID: 15919918
  75. suppresses autoimmune damage in relapsing-remitting, and non-remitting models of experimental autoimmune encephalomyelitis PMID: 16174101
  76. signaling of TRAIL-DR5 complexes requires ceramide-enriched membrane platforms PMID: 16636669
  77. neurotrophic factors have effects on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cells PMID: 16772303
  78. TRAIL deficiency might have an initial protective role but is not sufficient for the in vivo maintenance of long-term memory CD8 T cells in the absence of CD4 T help. PMID: 16818756
  79. prevention of experimental autoimmune encephalomyelitis by treatment with embryonic stem cell-derived dendritic cells(ES-DC)-TRAIL/myelin oligodendrocyte glycoprotein (MOG) is mediated by MOG-reactive CD4(+)CD25(+) Treg propagated by ES-DC-TRAIL/MOG PMID: 17202353
  80. led to decreased c-FLIP expression and allowed all MCL samples to undergo TRAIL-mediated apoptosis PMID: 17237443
  81. ability of TRAIL to modify inflammatory responses and to reduce neuronal cell death in meningitis suggests that it may be used as a novel antiinflammatory agent in invasive infections PMID: 17571163
  82. Tnfsf10 inhibits osteoclastic differentiation by counteracting RANKL-dependent p27Kip1 accumulation in pre-osteoclast precursors. PMID: 17620297
  83. Variants of human TRAIL (hTRAIL) and human CD95L (hCD95L), encompassing the TNF homology domain (THD), interact with the corresponding receptors and stimulate CD95 and TRAILR2 signaling after cross-linking. PMID: 17703232
  84. These findings suggest that the TRAIL/DR5 pathway is one of the mechanisms used by T regulatory cells to regulate immune responses both in vitro and in vivo. PMID: 17762882
  85. nuclear factor kappaB pathway inhibition blocks myeloma cell growth and induces apoptosis in strong synergy with TRAIL PMID: 17947462
  86. Results from this study suggest that osteoblasts containing intracellular S. aureus induce TRAIL expression in uninfected osteoblasts present in infected cultures. PMID: 18070069
  87. Local treatment with mTRAIL and CpG oligodeoxyribonucleotide can augment tumor antigen cross-presentation resulting in T-cell proliferation, enhanced cytotoxic T-lymphocyte activity, and increased animal survival in kidney cancer. PMID: 18089829
  88. pivotal role in cholestatic liver injury mediated by NK 1.1-positive NK/NKT cells PMID: 18220275
  89. CD4(+) T cell help for the CD8(+) T cell response is not contingent on the silencing of TRAIL expression and prevention of TRAIL-mediated apoptosis PMID: 18354179
  90. IL-15 codelivered with vaccines can overcome CD4+ helper T cell deficiency for induction of functionally efficient CD8+ T cells and maintenance of CD8+ cytotoxic T lymphocytes (CTLs) PMID: 18362335
  91. rpS6, especially in its unphosphorylated form, is a selective mediator of TRAIL-induced apoptosis PMID: 18362888
  92. Important component of immunity to influenza infections; TRAIL-mediated apoptosis is a mechanism used by influenza-specific CD8-positive T lymphocytes to eliminate influenza-infected cells and drive recovery from influenza. PMID: 18802095
  93. CD4+cd25+ Tregs control the TRAIL-dependent cytotoxicity of tumor-infiltrating DCs in rodent models of colon cancer. PMID: 18830416
  94. In situ infection of adenovirus expressing TRAIL or indoleamine 2, 3-dioxygenase, but not green fluorescent protein, significantly suppressed thyroiditis scores. PMID: 18988676
  95. Data demonstrate a key role for exudate macrophages in the induction of alveolar leakage and mortality in IV pneumonia, and suggest that epithelial cell apoptosis induced by TRAIL-expressing macrophages is a major underlying mechanism. PMID: 19064696
  96. Deficiency of GMDS leads to escape from natural killer cell-mediated tumor surveillance through modulation of TRAIL signaling. PMID: 19361506
  97. Results identify PACS-2 as an essential TRAIL effector, and show that Akt cooperates with 14-3-3 to regulate the homeostatic and apoptotic properties of PACS-2 that mediate TRAIL action. PMID: 19481529
  98. essential mechanism of NEMO-mediated protection of the liver by preventing NK cell tissue damage via TRAIL/DR5 signaling PMID: 19635861

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Subcellular Location Membrane, Single-pass type II membrane protein
Protein Families Tumor necrosis factor family
Tissue Specificity Widespread.
Database Links

KEGG: mmu:22035

STRING: 10090.ENSMUSP00000040271

UniGene: Mm.1062

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