Pig Ferritin heavy chain(FTH1) ELISA kit

Pig Ferritin heavy chain(FTH1) ELISA kit

Product Details

Citations

Customer Reviews and Q&A

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Product Details

Citations

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Customer Reviews and Q&A

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Pig Ferritin heavy chain(FTH1) ELISA kit

Citations (2) Protocol MSDS

Code	CSB-EL009030PI
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (2)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

ferritin, heavy polypeptide 1

Alternative Names

FTH1 ELISA Kit; FTHFerritin heavy chain ELISA Kit; Ferritin H subunit ELISA Kit; EC 1.16.3.1) [Cleaved into: Ferritin heavy chain ELISA Kit; N-terminally processed] ELISA Kit

Abbreviation

FTH1

Uniprot No.

P19130

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

4.7 ng/ml- 300 ng/ml

Sensitivity

1.175 ng/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig FTH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	89
	Range %	84-95
1:4	Average %	93
	Range %	89-98
1:8	Average %	99
	Range %	95-105

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

300

2.734

2.634

2.684

2.525

150

2.183

2.267

2.225

2.066

1.678

1.728

1.703

1.544

37.5

1.122

1.129

1.126

0.967

18.75

0.694

0.671

0.682

0.524

9.4

0.453

0.443

0.448

0.289

4.7

0.278

0.284

0.281

0.123

0.162

0.155

0.159

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Effects of natural oral alternatives to parental iron supplementation on haematological and health-related blood parameters of organic piglets E Merlot,animal,2024
Novel edible toys as iron carrier to prevent iron deficiency of postweaned pigs M.Anticoi,Animal,2021

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Function
(From Uniprot)

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.; (Microbial infection) Is unable to assume its function upon interaction with viral proteins, thereby increasing Fe concentration in the cytoplasm. This would inhibit the accumulation of reactive oxygen in host cells, leading to reduced apoptosis and increasing the survival of virus infected cell.

Subcellular Location

Cytoplasm.

Protein Families

Ferritin family

Database Links

KEGG: ssc:397030

UniGene: Ssc.23940

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.