Pig Interferon γ ,IFN-γ ELISA Kit

Instructions
Code CSB-E06794p
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interferon, gamma
Alternative Names IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
Abbreviation IFNG
Uniprot No. P17803
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %90-99
1:2Average %92
Range %85-99
1:4Average %95
Range %91-102
1:8Average %95
Range %90-100
Recovery
The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9586-102
EDTA plasma (n=4)9689-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.692 2.647 2.670 2.581
5002.404 2.465 2.435 2.346
2501.974 1.989 1.982 1.893
1251.523 1.541 1.532 1.443
62.50.945 0.942 0.944 0.855
31.20.613 0.635 0.624 0.535
15.60.337 0.324 0.331 0.242
00.090 0.087 0.089  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Gene References into Functions
  1. The regulatory effect of IFN-gamma on CYP3A29 expression is mediated via PXR. PMID: 25413352
  2. Mapping of QTL for mycoplasma and tetanus antibodies and IFNgamma. PMID: 20567833
  3. Data show taht the expression of PoIFN-gamma in insect cells was confirmed by Western Blot, indirect immunofluorescence assay and indirect ELISA. PMID: 17577980
  4. IFNgamma production from the frozen peripheral blood mononuclear cells was significantly higher than that from the fresh ones. PMID: 19766617
  5. Translational control of IL-18 expression by its 5'-UTR limits production of IL-18, resulting in restricted expression of mRNA and protein IFN-gamma in this model of crescentic glomerulonephritis(GN). Might amplify CD8+-mediated macrophage-dependent GN. PMID: 12787406
  6. selective changes in immature crypt cells induced by IFN-gamma bound to extracellular matrix could contribute to inappropriate responsiveness to commensal bacteria in inflammatory bowel diseases PMID: 15894119
  7. A functional single nucleotide polymorphism is reported in the coding region of IFN-gamma cDNA that markedly reduces antiviral activity of the IFN-gamma protein. PMID: 17416419
  8. The expression of IFN-gamma in recombiannt Lactococcus lactis as a tool for investigating downregulation of allergic predisposition of pigs to experimental food allergy is reported. PMID: 18164876

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Subcellular Location Secreted
Protein Families Type II (or gamma) interferon family
Tissue Specificity Released primarily from activated T lymphocytes.
Database Links

KEGG: ssc:396991

STRING: 9823.ENSSSCP00000000511

UniGene: Ssc.4093

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