Pig Interleukin 12,IL-12/P70 ELISA Kit

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Pig Interleukin 12,IL-12/P70 ELISA Kit

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Citations (1) Protocol MSDS

Code	CSB-E13288p
Size	96T,5×96T,10×96T
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Product Details
Citations (1)
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Customer Reviews and Q&A
Target Background

Target Name

Interleukin 12,IL-12/P70

Alternative Names

IL12A ELISA Kit; Interleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit

Abbreviation

IL12/P70

Uniprot No.

Q29053

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.45 ng/mL-30 ng/mL

Sensitivity

0.11 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	96
	Range %	91-101
1:4	Average %	84
	Range %	80-89
1:8	Average %	93
	Range %	88-97

Recovery

The recovery of pig IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-95
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.549	2.452	2.501	2.331
15	1.789	1.761	1.775	1.605
7.5	1.025	1.004	1.015	0.845
3.75	0.591	0.549	0.570	0.400
1.8	0.402	0.374	0.388	0.218
0.9	0.321	0.309	0.315	0.145
0.45	0.258	0.245	0.252	0.082
0	0.173	0.167	0.170

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Pig IL12/P70 ELISA Kit was designed for the quantitative measurement of Pig IL12/P70 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL .

Streptococcus suis Serotype 2 Infection Causes Host Immunomodulation Through Induction of Thymic Atrophy Wang S,Infect Immun,2020

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Subcellular Location

Secreted.

Protein Families

IL-6 superfamily

Database Links

KEGG: ssc:397053

STRING: 9823.ENSSSCP00000012501

UniGene: Ssc.13

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Related pathways

Jak-STAT signaling pathway Th1 and Th2 cell differentiation Toll-like receptor signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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