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Pig Interleukin 12,IL-12/P70 ELISA Kit

Instructions
Code CSB-E13288p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Interleukin 12,IL-12/P70
Alternative Names IL12A ELISA Kit; Interleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
Abbreviation IL12/P70
Uniprot No. Q29053
Species Sus scrofa (Pig)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.45 ng/mL-30 ng/mL
Sensitivity 0.11 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %92
Range %88-96
1:2Average %96
Range %91-101
1:4Average %84
Range %80-89
1:8Average %93
Range %88-97
Recovery
The recovery of pig IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9086-95
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
302.549 2.452 2.501 2.331
151.789 1.761 1.775 1.605
7.51.025 1.004 1.015 0.845
3.750.591 0.549 0.570 0.400
1.80.402 0.374 0.388 0.218
0.90.321 0.309 0.315 0.145
0.450.258 0.245 0.252 0.082
00.173 0.167 0.170 　
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

KEGG: ssc:397053

STRING: 9823.ENSSSCP00000012501

UniGene: Ssc.13
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