Pig Interleukin 18,IL-18 ELISA Kit

Code CSB-E06781p
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 18 (interferon-gamma-inducing factor)
Alternative Names
IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
Abbreviation
IL18
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %82-92
1:2Average %100
Range %95-105
1:4Average %91
Range %85-97
1:8Average %97
Range %91-103
Recovery
The recovery of pig IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8984-93
EDTA plasma (n=4)9491-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.562 2.687 2.625 2.443
10001.839 1.865 1.852 1.670
5001.104 1.135 1.120 0.938
2500.643 0.638 0.641 0.459
1250.412 0.435 0.424 0.242
62.50.321 0.341 0.331 0.149
31.250.262 0.248 0.255 0.073
00.181 0.183 0.182
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig IL18 ELISA Kit was designed for the quantitative measurement of Pig IL18 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

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Target Background

Function
(From Uniprot)
A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
Gene References into Functions
  1. Study reports that the monocyte-derived cytokines IL-12 and IL-18 act synergistically on porcine gammadelta T cells to induce IFN-gamma production. Additional stimuli such as the mitogen ConA and IL-2 are necessary to activate the cells for enhanced proliferation. PMID: 25036760
  2. IL-18 concentration in saliva was significantly increased during a 60-min acute immobilization stress in thirteen 5-month-old pigs. These results are the first evidence of a stress-related change of IL-18 in pig saliva. PMID: 21682650
  3. endometrial expression of CASP1 and IL18 associated with pregnancy establishment; alteration of CASP1 and IL18 following premature exposure of uterus to estrogen during early pregnancy may contribute to conceptus loss between Days 15 to 18 of pregnancy PMID: 20380728
  4. Porcine IL-18 regulates anti-pig cellular rejection in C57BL/6 mice. PMID: 17079874
Subcellular Location
Cytoplasm. Secreted.
Protein Families
IL-1 family
Database Links

KEGG: ssc:397057

STRING: 9823.ENSSSCP00000015962

UniGene: Ssc.20

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