Pig anti-Mullerian hormone (AMH) ELISA kit

Code CSB-E13408p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
anti-Mullerian hormone
Alternative Names
AMH ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
Abbreviation
AMH
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.25 ng/mL-50 ng/mL
Sensitivity
1.25 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 89-102  
1:2 Average % 93  
Range % 90-103  
1:4 Average % 90  
Range % 85-100  
1:8 Average % 93  
Range % 83-107  
Recovery
The recovery of pig AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-103  
EDTA plasma (n=4) 97 88-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
50 0.134 0.139 0.137    
25 0.227 0.238 0.233    
11.5 0.330 0.343 0.337    
3.75 0.611 0.592 0.602    
1.25 1.264 1.214 1.239    
0 2.686 2.635 2.661    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig AMH ELISA Kit was designed for the quantitative measurement of Pig AMH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.25 ng/mL-50 ng/mL and the sensitivity is 1.25 ng/mL.

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Target Background

Function
(From Uniprot)
This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
Subcellular Location
Secreted.
Protein Families
TGF-beta family
Database Links

UniGene: Ssc.97577

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