Pig apolipoprotein A1 (Apo-A1) ELISA Kit

Code CSB-E06834p
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name apolipoprotein A-I
Alternative Names APOA1Apolipoprotein A-I ELISA Kit; Apo-AI ELISA Kit; ApoA-I ELISA Kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA Kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA Kit
Abbreviation APOA1
Uniprot No. P18648
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 80 μg/mL-2000 μg/mL
Sensitivity 50 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %94
Range %89-103
1:2Average %90
Range %85-103
1:4Average %93
Range %88-100
1:8Average %89
Range %82-97
The recovery of pig Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9996-104
EDTA plasma (n=4)9086-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
20000.342 0.348 0.345
8000.533 0.483 0.508
4800.682 0.657 0.670
2001.021 0.987 1.004
801.267 1.325 1.296
01.903 1.954 1.929
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Gene References into Functions
  1. Alterations in the Apo A-I pattern is a good indicator of the presence and severity of infectious diseases in the pig.Lower overall amounts of Apo A-I were observed in Salmonella typhimurium and Escherichia coli infections. PMID: 25377528
  2. blood level decreases during experimentally induced acute-phase processes in pigs PMID: 15845530
  3. down-regulation of apolipoprotein A-I and A-IV messages in the liver may be mediated by interleukin 6 and tumor necrosis factor-alpha PMID: 15878672
Subcellular Location Secreted
Protein Families Apolipoprotein A1/A4/E family
Tissue Specificity Major protein of plasma HDL, also found in chylomicrons. Synthesized predominantly in the intestine and the liver.
Database Links

KEGG: ssc:397691

STRING: 9823.ENSSSCP00000015994

UniGene: Ssc.807

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