Pig apolipoprotein E (Apo-E) ELISA Kit

Code CSB-E17889p
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Pig APOE ELISA Kit was designed for the quantitative measurement of Pig APOE protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.81 ng/mL.

Target Name apolipoprotein E (Apo-E)
Alternative Names APOEApolipoprotein E ELISA kit; Apo-E ELISA kit
Abbreviation APOE
Uniprot No. P18650
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 ng/mL-2000 ng/mL
Sensitivity 7.81 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 96
Range % 85-105
1:200 Average % 90
Range % 89-98
1:400 Average % 96
Range % 82-103
1:800 Average % 97
Range % 92-99
Recovery
The recovery of pig Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 92-105
EDTA plasma (n=4) 95 85-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average  
2000 0.131 0.134 0.133  
1000 0.223 0.226 0.225  
500 0.335 0.341 0.338  
250 0.562 0.553 0.558  
125 0.862 0.860 0.861  
62.5 1.192 1.214 1.203  
31.25 1.589 1.607 1.598  
0 2.417 2.395 2.406  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells.
Gene References into Functions
  1. These data suggest that the -155T>A mutation in the promoter region of the porcine APOE gene is an important functional variant PMID: 21549015
Subcellular Location Secreted. Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
Protein Families Apolipoprotein A1/A4/E family
Database Links

KEGG: ssc:397576

STRING: 9823.ENSSSCP00000003343

UniGene: Ssc.1342

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