Pig coagulation factor VIII(FVIII) ELISA Kit

Pig coagulation factor VIII(FVIII) ELISA Kit

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Product Details

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Pig coagulation factor VIII(FVIII) ELISA Kit

Protocol

Code	CSB-E15000p
Size	96T,5×96T,10×96T
Price	Request a Quote
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Alternative Names

F8 ELISA kit; CF8Coagulation factor VIII ELISA kit; Procoagulant component ELISA kit

Abbreviation

Uniprot No.

P12263

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

62.5 ng/mL-4000 ng/mL

Sensitivity

15.62 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig FVIII in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	89
	Range %	84-95
1:200	Average %	94
	Range %	89-99
1:400	Average %	93
	Range %	86-100
1:800	Average %	98
	Range %	91-103

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

4000

0.134

0.128

0.131

2000

0.221

0.237

0.229

1000

0.384

0.398

0.391

500

0.615

0.629

0.622

250

0.868

0.881

0.875

125

1.214

1.253

1.234

62.5

1.649

1.684

1.667

2.236

2.125

2.181

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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Function
(From Uniprot)

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.

Gene References into Functions

It was concluded that VEGF and factor VIII are important growth factors associated with fetal development in pigs and are identified in all uterine segments. PMID: 28237345
Report Factor VIII organisation on nanodiscs with different lipid composition. PMID: 25589466
thrombin stimulates transglutaminase activity in articular cartilage by directly cleaving factor XIII and by receptor-mediated up-regulation of factor XIII synthesis PMID: 14983233
cupredoxin-like A1 subdomains in fVIII contain inter-species differences that are a result of selective pressure on the dissociation rate constant PMID: 16513639

Subcellular Location

Secreted, extracellular space.

Protein Families

Multicopper oxidase family

Database Links

KEGG: ssc:397339

STRING: 9823.ENSSSCP00000028556

UniGene: Ssc.16043

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Related pathways

Complement and coagulation cascades

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.