Pig glucagon like peptide 1(GLP1) ELISA Kit

Code CSB-EQ027281PI
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
glucagon like peptide 1(GLP1)
Abbreviation
GLP1
Species
Sus scrofa (Pig)
Sample Types
serum, plasma
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig GLP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-93
1:2Average %95
Range %90-105
1:4Average %98
Range %92-110
1:8Average %93
Range %85-98
Recovery
The recovery of pig GLP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-104
EDTA plasma (n=4)9490-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.028 2.014 2.021 1.803
5001.562 1.540 1.551 1.333
2500.949 0.932 0.941 0.723
1250.565 0.568 0.567 0.349
62.50.351 0.347 0.349 0.131
31.20.301 0.322 0.312 0.094
15.60.284 0.286 0.285 0.067
00.214 0.222 0.218  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig GLP1 ELISA Kit was designed for the quantitative measurement of Pig GLP1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

 Q&A
Q:

I want to measure total GLP-1 in plasma samples, but I do not understand how it would be biologically relevant to measure 1-37 (with no cross reactivity to other fragments) since GLP-1 is released from the L-cells to the blood stream as either 7-37 or 7-36 and then rapidly degraded to 9-37 and 9-36.
As far as I understand 1-37 is not present in plasma. Could you please explain?

A:
Thanks for your mail!
For the in-body enviroment, the half life of GLP-1 is around 30 seconds. Then GLP-1 cannot be detected in this enviroment.
When the sample prepared and detached from the original in-body enviroment, it can be detected in this condition.
As for the different fragments, it is validated from our side that there is no across activity with another peptide fragments.
Q:

I was wondering if you knew whether the "Pig glucagon like peptide 1(GLP1) ELISA Kit" is meant for pig serum/plasma, or human serum/plasma?

A:
Thanks for your inquir! Pig glucagon like peptide 1(GLP1) ELISA KitCSB-EQ027281PI
This kit is specific to pig. It is suitable to test pig serum/plasma.
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X