| Target Name |
glucagon-like peptide-1,GLP-1 |
| Alternative Names |
N/A |
| Abbreviation |
Glp1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
0.625 pg/mL-40 pg/mL |
| Sensitivity |
0.156 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:100 | Average % | 86 | | | Range % | 84-95 | | | 1:200 | Average % | 95 | | | Range % | 91-100 | | | 1:400 | Average % | 84 | | | Range % | 81-89 | | | 1:800 | Average % | 92 | | | Range % | 86-98 | |
|
| Recovery |
| The recovery of rat GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 91 | 87-96 | | | EDTA plasma (n=4) | 103 | 95-108 | | | | | | | | | | | | | | | | | |
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.607 | 2.744 | 2.676 | 2.531 | | | 20 | 2.303 | 2.451 | 2.377 | 2.232 | | | 10 | 1.817 | 1.922 | 1.870 | 1.725 | | | 5 | 1.133 | 1.191 | 1.162 | 1.017 | | | 2.5 | 0.621 | 0.632 | 0.627 | 0.482 | | | 1.25 | 0.344 | 0.355 | 0.350 | 0.205 | | | 0.625 | 0.234 | 0.248 | 0.241 | 0.096 | | | 0 | 0.140 | 0.150 | 0.145 | | |
|
|
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
