Rat glucagon-like peptide-1,GLP-1 ELISA Kit

Code CSB-E08117r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name glucagon-like peptide-1,GLP-1
Alternative Names N/A
Abbreviation Glp1
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.625 pg/mL-40 pg/mL
Sensitivity 0.156 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:100Average %86
Range %84-95
1:200Average %95
Range %91-100
1:400Average %84
Range %81-89
1:800Average %92
Range %86-98
The recovery of rat GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9187-96
EDTA plasma (n=4)10395-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
402.607 2.744 2.676 2.531
202.303 2.451 2.377 2.232
101.817 1.922 1.870 1.725
51.133 1.191 1.162 1.017
2.50.621 0.632 0.627 0.482
1.250.344 0.355 0.350 0.205
0.6250.234 0.248 0.241 0.096
00.140 0.150 0.145  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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