Pig interleukin 4,IL-4 ELISA Kit

Pig interleukin 4,IL-4 ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

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Pig interleukin 4,IL-4 ELISA Kit

Citations (4) Protocol MSDS

Code	CSB-E06785p
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details
Citations (4)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

interleukin 4

Alternative Names

IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit

Abbreviation

IL4

Uniprot No.

Q04745

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	90
	Range %	85-95
1:4	Average %	89
	Range %	84-93
1:8	Average %	96
	Range %	92-101

Recovery

The recovery of pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	92	86-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.719	2.652	2.686	2.492
200	1.962	1.861	1.912	1.718
100	1.213	1.204	1.209	1.015
50	0.748	0.689	0.719	0.525
25	0.551	0.527	0.539	0.345
12.5	0.355	0.374	0.365	0.171
6.25	0.295	0.272	0.284	0.090
0	0.192	0.196	0.194

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Pig IL4 ELISA Kit was designed for the quantitative measurement of Pig IL4 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

Effects of GABA Supplementation on Intestinal SIgA Secretion and Gut Microbiota in the Healthy and ETEC-Infected Weanling Piglets Y Zhao,Mediators of Inflammation,2020
Streptococcus suis Serotype 2 Infection Causes Host Immunomodulation Through Induction of Thymic Atrophy Wang S,Infect Immun,2020
Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets Yinghui Li, et al,Journal of Functional Foods,2019
Effects of Selective Inhibition of PDE4 by YM976 on Airway Reactivity and Cough in Ovalbumin-Sensitized Guinea Pigs Mokr J.et al,Adv Exp Med Biol.,2016

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Function
(From Uniprot)

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

Gene References into Functions

IL-4- and IL-10-producing invariant NKT cells inhibit the Th1 cell response, but not the Th17 cell response PMID: 21572032
IL-4 induced activation of Akt/SREBP-1/lipid biosynthesis in EC, resulting in protection against membrane attack complex and melittin, in association with mitochondrial protection. PMID: 20017192
There was no significant difference of IL-4 production between fresh and frozen peripheral blood mononuclear cells. PMID: 19766617
The endothelium and subendothelium of porcine iliac arteries that are transduced with recombinant pig IL-4 are effectively protected from complement-dependent immediate injury after perfusion with human blood. PMID: 17082655
unlike in humans and mice, porcine IL-4 blocks antibody and IL-6 secretion and suppresses antigen-stimulated proliferation of B cells PMID: 18761033

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Subcellular Location

Secreted.

Protein Families

IL-4/IL-13 family

Database Links

KEGG: ssc:397225

STRING: 9823.ENSSSCP00000015193

UniGene: Ssc.15837

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Service

Phage Display Service

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Protein Service

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Oligo Synthesis Service

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Related pathways

Jak-STAT signaling pathway PI3K-Akt signaling pathway Fc epsilon RI signaling pathway Hematopoietic cell lineage IL-17 signaling pathway T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.