Pig neutrophil gelatinase-associated lipocalin(NGAL)ELISA Kit

Code CSB-E16507p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
neutrophil gelatinase-associated lipocalin(NGAL)
Abbreviation
NGAL
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 105
Range % 101-108
1:400 Average % 96
Range % 92-98
1:800 Average % 96
Range % 90-99
1:1600 Average % 89
Range % 84-93
Recovery
The recovery of pig NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-94
EDTA plasma (n=4) 99 99-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average
500 0.132 0.135 0.134
250 0.254 0.265 0.260
125 0.612 0.563 0.588
62.5 1.054 1.068 1.061
31.2 1.538 1.652 1.595
15.6 2.079 2.122 2.101
7.8 2.435 2.454 2.445
0 2.841 2.878 2.860
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig NGAL ELISA Kit was designed for the quantitative measurement of Pig NGAL protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Citations

Customer Reviews and Q&A

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 Q&A
Q:

I would like to be sure it is good both for urine and serum. Could you confirm?

A:
Thanks for your inquiry. We tested serum and plasma samples under 1:500 dilution before and the test value is 30--125pg/ml with this kit CSB-E16507p.
The final concentration is 15--62.5ng/ml. We suggest you order 24T sample kit and do a pretest first if you want to test urine samples. Pls let me know if you have any further questions. Thank you.
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