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Pig neutrophil gelatinase-associated lipocalin(NGAL)ELISA Kit

Pig neutrophil gelatinase-associated lipocalin(NGAL)ELISA Kit

Citations (2) Protocol

Code	CSB-E16507p
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (2)
Customer Reviews and Q&A

Product Details

Target Name

neutrophil gelatinase-associated lipocalin(NGAL)

Abbreviation

NGAL

Uniprot No.

F1RRX1

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	105
	Range %	101-108
1:400	Average %	96
	Range %	92-98
1:800	Average %	96
	Range %	90-99
1:1600	Average %	89
	Range %	84-93

Recovery

The recovery of pig NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-94
EDTA plasma (n=4)	99	99-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
500	0.132	0.135	0.134
250	0.254	0.265	0.260
125	0.612	0.563	0.588
62.5	1.054	1.068	1.061
31.2	1.538	1.652	1.595
15.6	2.079	2.122	2.101
7.8	2.435	2.454	2.445
0	2.841	2.878	2.860

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Pig NGAL ELISA Kit was designed for the quantitative measurement of Pig NGAL protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Citations

Renal hemodynamics by return cannular position of extracorporeal membrane oxygenation in swine Kim H J, et al,Journal of Thoracic Disease,2019
Physiological effects of altering oxygenation during kidney normothermic machine perfusion Adams TD, et al,American Journal Of Physiology-Renal Physiology,2019

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

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■ Q&A

I would like to be sure it is good both for urine and serum. Could you confirm?

Thanks for your inquiry. We tested serum and plasma samples under 1:500 dilution before and the test value is 30--125pg/ml with this kit CSB-E16507p.
The final concentration is 15--62.5ng/ml. We suggest you order 24T sample kit and do a pretest first if you want to test urine samples. Pls let me know if you have any further questions. Thank you.

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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