Pig prolactin (PRL) ELISA Kit

Pig prolactin (PRL) ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

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Product Details

Citations

Related Products

Customer Reviews and Q&A

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Pig prolactin (PRL) ELISA Kit

Citations (5) Protocol MSDS

Code	CSB-E15801p
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (5)
Related Products
Customer Reviews and Q&A
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Alternative Names

PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit

Abbreviation

PRL

Uniprot No.

P01238

Species

Sus scrofa (Pig)

Sample Types

serum, plasma

Detection Range

0.78 ng/ml-50 ng/ml.

Sensitivity

0.195 ng/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	87-95
1:2	Average %	94
	Range %	89-97
1:4	Average %	99
	Range %	94-102
1:8	Average %	95
	Range %	91-99

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2.677

2.713

2.695

2.495

1.886

1.901

1.894

1.694

12.5

1.365

1.412

1.389

1.189

6.25

1.000

1.032

1.016

0.816

3.12

0.785

0.792

0.789

0.589

1.56

0.546

0.554

0.550

0.350

0.78

0.346

0.324

0.335

0.135

0.199

0.200

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Pig PRL ELISA Kit was designed for the quantitative measurement of Pig PRL protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.78 pg/mL-50 pg/mL and the sensitivity is 0.195 pg/mL .

Impacts of hybrid rye and fiber level in the diet of pregnant and early lactation sows on the reproductive performance and fecal microbiota of sows and their piglets L Mazroua, L Lo Verso, J Dallaire,Canadian Journal of Animal Science,2025
Impacts du seigle hybride dans l'alimentation des truies en gestation et en début de lactation sur les performances de reproduction et le microbiote intestinal des truies et leurs porcelets L Mazroua,Thesis&Dissertation,2024
Valine supplementation during late pregnancy in gilts increases colostral protein synthesis through stimulating mTOR signaling pathway in mammary cells Long Che, et al,Amino Acids,2019
Effects of maternal alpha-ketoglutarate supplementation during lactation on the performance of lactating sows and suckling piglets Jiang Q, et al,Archives of Animal Nutrition,2019
Effect of optimizing dietary valine-to-lysine ratio in late gestation or lactation on biochemical indices and performance of lactating primiparous sows KaiguoGao,Animal Feed Science and Technology,2019

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Function
(From Uniprot)

Prolactin acts primarily on the mammary gland by promoting lactation.

Gene References into Functions

Prolactin and, to a lesser extent progesterone, which increase in early pregnancy, enhance basal and glucose-stimulated insulin secretion in part by increasing glucokinase activity and amplifying cAMP levels. PMID: 14559722
iv infusion of prolactin primarily caused coronary, mesenteric, renal, and iliac vasoconstriction PMID: 17463060
despite a lack of clear relationship between PRL/C499T polymorphism in 5' UTR of the PRL gene and plasma prolactin cioncentration, gene polymorphism impact on reproductiove performance cannot be excluded PMID: 19675522

Subcellular Location

Secreted.

Protein Families

Somatotropin/prolactin family

Database Links

KEGG: ssc:396965

STRING: 9823.ENSSSCP00000001152

UniGene: Ssc.8204

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Related pathways

Jak-STAT signaling pathway PI3K-Akt signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.